Fig. 1
Twenty percent OLVafter serum induced the most obvious inflammation and oxidative stress in A549 cells. A Representative image of cell morphology after treatment with different concentrations of serum. Scale bar = 100 μm. B Western blot analysis images of IL-6 in each group. C The relative protein levels of IL-6 were calculated. D qRT–PCR analysis of IL-6 in each group. E ROS levels were measured by DCFH-DA staining, and the relative DCF-DA fluorescence intensities were normalized and summarized. Scale bar = 100 μm. The results are shown as the means ± SD of 3 individual experiments. *P < 0.05; **P < 0.01; ***P < 0.001