Fig. 5
Prdx6 inhibits activation of the NF-κB signaling pathway by suppressing the nuclear translocation of P65. A Immunofluorescence analysis of P65 in cells transfected with either Prdx6-siRNA or NC-siRNA. DAPI is shown in blue and represents the nucleus. Scale bar = 100 μm. B Nuclear (N) and cytosolic/membrane (C + M) proteins of the NF-κB pathway in Prdx6-siRNA- or NC-siRNA-transfected cells. The cells in all groups were first pretreated with 40 μg/ml curcumin and DMSO and then changed to 20% OLVafter serum for 48 h. The results are shown as the means ± SD of 3 individual experiments. *P < 0.05; **P < 0.01; ***P < 0.001