Fig. 2

Overexpression of H19 inhibits apoptosis and inflammation in lungs of mice with sepsis-induced ALI. A The lung tissue injury was assessed by TUNEL staining, N = 6; B the levels of Bax and Bcl-2 were measured by Western blot, N = 6; C the total protein concentration was measured and the number of total cells, neutrophils and macrophages in BALF of mice was counted, N = 12; D, E the levels of inflammatory cytokines (TNF-α, IL-1β, IL-6, and IL-10) in BALF (N = 12) and lung tissues (N = 6) of mice were determined by ELISA. Data were presented as mean ± standard deviation. Comparisons among multi-groups were made using one-way ANOVA and Tukey’s multiple comparisons test. *p < 0.05, **p < 0.01, ***p < 0.001