Fig. 3

Overexpression of H19 suppresses apoptosis of PMVECs and release of pro-inflammatory cytokines induced by LPS. The in vitro model of sepsis-induced ALI was established using LPS-induced PMVECs and H19 was overexpressed via transfection. A The relative expression pattern of H19 was determined by RT-qPCR; B the apoptotic rate of LPS-induced PMVECs was estimated by flow cytometry; C the relative expression levels of Bax and Bcl-2 were measured by Western blot; D the levels of TNF-α, IL-1β, IL-6 and IL-10 in PMVECs were measured by ELISA. Cell experiments were repeated 3 times. Data were presented as mean ± standard deviation. Comparisons among multi-groups were made using one-way ANOVA and Tukey’s multiple comparisons test. **p < 0.01, ***p < 0.001