Table 2 Sequence of real-time qPCR primers of stemness gene

β-actin

F

5′- CATGTACGTTGCTATCCAGGC -3′

R

5′- CTCCTTAATGTCACGCACGAT -3′

SOX9

F

5′- AGCGAACGCACATCAAGAC -3′

R

5′- CTGTAGGCGATCTGTTGGGG -3′

SOX2

F

5′- GTTCTAGTGGTACGGTAGGAGCTTTG -3′

R

5′- TTTGATTGCCATGTTTATCTCGAT -3′

Nanog

F

5′- CCAGCTGTGTGTACTCAATGATAGATTT -3′

R

5′- TTCTGCCACCTCTTAGATTTCATTC -3′

SOX4

F

5′- AGCGACAAGATCCCTTTCATTC -3′

R

5′- CGTTGCCGGACTTCACCTT -3′

PD-L1

F

5′- GCTGCACTAATTGTCTATTGGGA -3′

R

5′- AATTCGCTTGTAGTCGGCACC -3′

Krt5

F

5′-AGGAGTTGGACCAGTCAACAT -3′

R

5′- TGGAGTAGTAGCTTCCACTGC-3′

Krt14

F

5′-TGAGCCGCATTCTGAACGAG-3′

R

5′-GATGACTGCGATCCAGAGGA-3′