Fig. 1

N6-methyladenosine modification and the protein expression of FRAS1 was increased in NSCLC tumor tissues and cell lines. A Venn diagram showed the intersection of the co-related genes with METTL3 and the co-related genes with YTHDF1 in lung cancer. There are six genes in the intersection list. FRAS1, PLA2G3, BTNL2, RPAP3, MS4A2, and TAGLN. B The mRNA level of FRAS1 was analyzed in paired NSCLC tumors tissues and normal tissues (n = 106) via TCGA. C The overall survival of NSCLC patients with FRAS1 expression was analyzed by using Kaplan–Meier plotter. Hazard ratio (HR) = 1.24, 95% Confidence Interval (CI) = 1.1–1.41, log rank P = 7e-04. D FRAS1 expression at mRNA level was analyzed by qRT-PCR in five pairs of LUAD samples. N: Normal tissues. T: Tumor tissues. E The m6A level of FRAS1 was determined by using MeRIP-qPCR in five pairs of LUAD samples. F FRAS1 protein level was detected by western blot in five pairs of LUAD samples. G FRAS1 expression at mRNA level was analyzed by qRT-PCR in NSCLC cell lines including A549, HCC827, NCI-H1975, and NCI-H1299, and lung epithelial cell line HBEC. H The m6A level of FRAS1 was analyzed in the above five cell lines. I FRAS1 expression at protein level was detected by western blot in A549, HCC827, NCI-H1975 and NCI-H1299, and HBEC. **P < 0.01, ***P < 0.001 indicates a significant difference between the indicated groups. NS, not significant