Fig. 2

The FRAS1 m6A methylation site occurs near the terminator codon in NSCLC. A The mRNA level of METTL3 in NSCLC was analyzed using UALCAN (http://ualcan.path.uab.edu/). B The predicted m6A motifs in the coding sequence (CDS) of FRAS1. Two PCR primers designed for different FRAS1 DNA segments, as marked by PCR 1 and PCR 2. C FRAS1 DNA fragments were amplified by qRT-PCR after m6A immunoprecipitation using m6A antibody in HCC827 and H1975 cells, which was normalized to IgG control of HCC827. D The m6A level was detected in METTL3-knockdown HCC827 and NCI-H1975 cells by qRT-PCR after m6A immunoprecipitation. E The knockdown efficiency of shMETTL3 was evaluated by qRT-PCR and western blot. Values are represented as means ± SD. *P < 0.05 or **P < 0.01 or ***P < 0.001 indicates a significant difference between the indicated groups. NS, not significant