Fig. 4

YTHDF1 keeps FRAS1 mRNA stability. A The mRNA level of YTHDF1 was analyzed in NSCLC via UALCAN. B The FRAS1 enrichment in RIP assay was performed using YTHDF1 antibody and quantified by qPCR. The relative FRAS1 enrichment was normalized to input. C The relative expression of YTHDF1 and FRAS1 at mRNA level was determined by qRT-PCR in HCC827 and NCI-H1975 cells with YTHDF1 silence (n = 3, left). **P < 0.01. The knockdown efficiency of shYTHDF1 was evaluated by western blot (right). D shYTHDF1 stable cells were transfected with plasmid Myc-RPL22 for 48 h. Ribosomal immunoprecipitation was performed using antibody Myc. The realtive FRAS1 expression was quantified by qPCR. E The mRNA level of FRAS1 was analyzed in shYTHDF1 stable cells with treatment of actinomycin D (5 μg/ml, left). The protein level of FRAS1 was detected in shYTHDF1 cells with or without MG132 (1 μM) for 24 h. GAPDH was used as an internal control. Values are represented as means ± SD. *P < 0.05 or **P < 0.01 or ***P < 0.001 indicates a significant difference between the indicated groups. ns, not significant