Fig. 1

Experimental design and raw data acquisition. A Schematic representation of a murine model of inflammatory lung injury. Mice are administered intratracheal heat-killed methicillin resistant staph aureus (MRSA) or saline control. After 18 h animals are anesthetized, intubated and initiated on mechanical ventilation. A left lateral thoracotomy is performed to expose the lung surface which is overlayed with a custom made “lung window” (top right) equipped with suction and overlying coverslip. Two-photon intravital microscopy is performed to generate images in control or MRSA injured animals (B–C)