Fig. 4

Knockdown of miR-125b-5p protects mice from the development of asthma via relieving M2 polarization in macrophages. A The histopathology of asthmatic inflammation in WT or KD mice stimulated by Der f1 were evaluated using H&E staining (magnification 400 × and 200 × , scale bar: 200 μm). B The CD206 and iNOS of macrophages from BALF of WT and miR-125b-5p KD mice which treatment with Der f1 was analyzed by flow cytometry. C Percentage of macrophages cells in B. D The expression levels of M1 associated genes and M2 associated genes from BALF were quantified using RT-PCR. Data are mean ± SD from three independent experiments. n = 5–6 mice for each group. *P < 0.05, **P < 0.01, ***P < 0.001