Fig. 2
From: Antifibrotic mechanism of avitinib in bleomycin-induced pulmonary fibrosis in mice
Avitinib slows TGF-β1-induced migration of NIH-3T3 cells in vitro. A MTT assays of NIH-3T3 cells. Cells were exposed to the indicated doses of AVB (0.08 to 20 µM) for 24 h. B IC50 = 6.857 µM. C CAGA-mouse embryonic fibroblast NIH-3T3 cells were treated with TGF-β1 (5 ng/mL) and a series concentration (0.16, 0.31, 0.63, 1.25, 2.5 µM) of AVB for 18 h. Quantitative analyses are shown beside.D TGF- β1 (5 ng/mL) and/or AVB (1.25, 2.5 µM) incubated with NIH-3T3 cells for 0 h, 12 h, 24 h. Scale bars: 100 μm.(E) Distance analyses are shown below. Data was noted as the means ± SD, n = 3. #P < 0.05, ###P < 0.001, ####P < 0.0001 versus Control group. *P < 0.05, **P < 0.01, ****P < 0.0001 versus TGF-β1 group