Fig. 4

Avitinib inhibits bleomycin-induced fibroblasts activation and Extracellular matrix (ECM) production in vivo. BLM (2U/kg) was intratracheally given at day 0, and Nin (100 mg/kg) and AVB (15, 30 and 60 mk/kg) were orally treated at day 7–13 and harvested at day 14. A The expression levels of Col 1 and α-SMA were analyzed by immunohistochemistry in left lung sections. Scale bars: 50 μM. B-C The mRNA translation levels of Col 1 and α-SMA in lung tissue homogenates. D-F Western Blot was used to analyze the protein expression levels of Col 1 and α-SMA in lung tissue homogenates. Densitometric analyses are shown beside. Data was noted as the means ± SD, n = 6. ^###P < 0.001, ^####P < 0.0001 versus NaCl group. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 versus BLM group