Fig. 10

LTBP1 was upregulated in monocrotaline (MCT)-induced PAH and (PDGF-BB)-induced PASMCs. A, B mPAP and RVHI were increased in rats of MCT-PAH. C HE staining of the lung in MCT-induced PAH rats and control (Scale bar = 25 μ m). D, E WT% and WA % of pulmonary arteries were calculated from HE staining. F Western blotting and quantification of LTBP1 and β-actin in the lungs of (MCT)-induced PAH (n = 9) and control (n = 8). G Immunohistochemistry analysis of LTBP1 in lungs, Scale bar = 50 μm. H Immunofluorescence of lung tissues with LTBP1 (green) and CD4 (red); Scale bar = 50 μm; I Western blot and quantification of LTBP1 and β-actin in the (PDGF-BB)-induced PASMCs and control, n = 4. Data represent the mean ± SD and Student t-test was used to compare the two groups. ****: P < 0.0001 vs Ctrl; *: P < 0.05 vs Ctrl. MCT: monocrotaline; Ctrl: control; MCT-PAH: MCT-induced pulmonary arterial hypertension