Fig. 3
TMP pretreatment mitigated Golgi stress and activated the Nrf2/HO-1 pathway in the LPS-stimulated lung tissues of mice. A Immunofluorescence assays of GM130 protein by fluorescence microscope (original magnification, × 400). Scale bar: 100 μm. Red stood was used for FITC-GM130 stained sections, and blue stood was used for DAPI-stained nuclear structure. B-H Bands and semiquantification of western blotting to evaluate the expression of Golgi stress-related (GM130, Golgin97, ATP2C1, and GOLPH3) and pathway-related (Nrf2 and HO-1) proteins in the lung tissues of mice (n = 3 mice/group). The blots were cropped for improving the clarity and conciseness of the presentation. Full-length blots were presented in Additional file 1. Band intensity analysis on western blotting images shows their relative ratio to β-actin. Values from three independent samples are expressed as mean ± SEM, and multiple comparisons were performed using one-way ANOVA with the Bonferroni coefficient. *Significant difference compared with the control group, *P < 0.05; #Significant difference compared with the LPS group, P < 0.05. ANOVA: analysis of variance; DAPI: 6-diamino-2-phenylindole; HO-1: heme oxygenase-1; LPS: lipopolysaccharide; Nrf2: nuclear factor-erythroid 2-related factor 2; SEM: standard error of the mean; TMP: tetramethylpyrazine