Fig. 5

Blocking of the Nrf2/HO-1 pathway by Nrf2 KO partially counteracted the effects of TMP-afforded preservation of Golgi stress in endotoxin-induced ALI. A Immunofluorescence staining to observe GM130 protein expression in lung tissue (original magnification, × 400). Scale bar: 100 μm. B-H Bands and semi-quantification of western blotting to evaluate the expression of Golgi stress-related (GM130, Golgin97, ATP2C1, and GOLPH3) and pathway-related (Nrf2 and HO-1) proteins of lung tissues in mice. The blots were cropped for improving the clarity and conciseness of the presentation. Full-length blots were presented in Additional file 2. Band intensity analysis on western blotting images shows their relative ratio to β-actin. Values from three independent samples are presented as mean ± SEM, and multiple comparisons were performed using one-way ANOVA with the Bonferroni coefficient. ns = no statistical difference, ^* P < 0.05, ^** P < 0.01, ^*** P < 0.001. ANOVA: analysis of variance; HO-1: heme oxygenase-1; KO: knockout; Nrf2: nuclear factor-erythroid 2-related factor 2; SEM: standard error of the mean; TMP: tetramethylpyrazine; WT: wild-type