Fig. 6

Nrf2 knockdown counteracted the effects of TMP-mitigated LPS-stimulated inflammation and oxidative damage in MLE12 cells. MLE12 cells were incubated with different LPS concentrations for 24 h, followed by a CCK-8 assay to determine cell viability. Data from five individual experiments were analyzed using one-way ANOVA and Bonferroni correction. Significant differences from the LPS = 0 group: ^* P < 0.05, ^** P < 0.01, ***P < 0.001. B CCK-8 assay was used to analyze the viability of MLE12 cells cultivated with different TMP concentrations prior to LPS treatment. Data from five individual experiments were analyzed using a one-way ANOVA and Bonferroni correction. Significant difference from TMP = 0 and LPS = 0 group: ^* P < 0.05. Significant difference from TMP = 0 and LPS 10 ug/ml group: ^# P < 0.05. C, D Levels of proinflammatory factors IL-1β and IL-6 in the cell supernatant in each group. E, F The levels of MDA and SOD activity indicated the oxidative stress status of each group. Data in (C)-(F) are expressed as mean ± SEM, and multiple comparisons were performed using one-way ANOVA with the Bonferroni coefficient (n = 6). *Significant difference compared with the control group, P < 0.05; ^#Significant difference compared with the LPS group, P < 0.05; ^&Significant difference from the LPS + TMP group, P < 0.05. ANOVA: analysis of variance; CCK-8: Cell Counting Kit-8; HO-1, heme oxygenase-1; KO, knockout; LPS, lipopolysaccharide; MDA: malondialdehyde; Nrf2: nuclear factor-erythroid 2-related factor 2; SOD: superoxide dismutase; TMP, tetramethylpyrazine