Fig. 7

Nrf2 knockdown partially counteracted the Golgi stress-alleviating effect of TMP in LPS-stimulated MLE12 alveolar epithelial cells. A-G Representative western blotting and semi-quantification of Golgi stress-related (GM130, Golgin97, ATP2C1, and GOLPH3) and pathway-related (Nrf2 and HO-1) proteins. The blots were cropped for improving the clarity and conciseness of the presentation. Full-length blots were presented in Additional file 3. Band intensity analysis on western blotting images shows their relative ratio to β-actin. Data were expressed as mean ± SEM, and multiple comparisons were performed using one-way ANOVA with the Bonferroni coefficient (n = 3). *Significant difference compared with the control group, P < 0.05; ^#Significant difference compared with the LPS group, P < 0.05; ^&Significant difference from the LPS + TMP group, P < 0.05. ANOVA: analysis of variance; HO-1, heme oxygenase-1; LPS: lipopolysaccharide; Nrf2: nuclear factor-erythroid 2-related factor 2; SEM: standard error of the mean; TMP: tetramethylpyrazine