Table 1 Animal experimental treatments and groupings

Effects of TMP on LPS-induced ALI in C57BL/6 J mice (n = 6/group)

 Control

Sham operation plus normal saline

 LPS

Caudal vein injection of 15 mg/kg LPS diluted in 0.2 ml saline for 12 h

 LPS + TMP

50 mg/kg TMP was pretreated intraperitoneally (i.p.) 1 h prior to LPS challenge

 TMP

Sham operation plus 50 mg/kg TMP

Effects of Nrf2/HO-1 pathway on Golgi stress during TMP attenuates lung injury in Nrf2 knockout (Nrf2 KO) and WT mice (n = 6/group)

 WT + Control

WT mice + Sham operation plus normal saline

 Nrf2 KO + Control

Nrf2 KO mice + Sham operation plus normal saline

 WT + LPS

WT mice + Caudal vein injection of 15 mg/kg LPS diluted in 0.2 ml saline for 12 h

 Nrf2 KO + LPS

Nrf2 KO mice + Caudal vein injection of 15 mg/kg LPS diluted in 0.2 ml saline for 12 h

 WT + LPS + TMP

WT mice + 50 mg/kg TMP were intraperitoneally (i.p.) administered 1 h prior to LPS challenge

 Nrf2 KO + LPS + TMP

Nrf2 KO mice + 50 mg/kg TMP was intraperitoneally (i.p.) administered 1 h prior to LPS challenge