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Table 2 Cell experimental treatments and groupings

From: Tetramethylpyrazine ameliorates endotoxin-induced acute lung injury by relieving Golgi stress via the Nrf2/HO-1 signaling pathway

Grouping

Treatment

Effects of the Nrf2/HO-1 pathway on TMP-mediated Golgi stress during LPS-induced oxidative injury in Nrf2 siRNA and NC siRNA-transfected MLE12 cells (n = 6 per group)

 Control

Cells are cultured normally in medium

 LPS

5 μg/ml LPS incubated for 24 h

 LPS + TMP

50 μg/ml TMP pre-incubated 1 h prior to 5 μg/ml LPS for 24 h

 Nrf2 siRNA + LPS + TMP

50 μg/ml TMP pre-incubated 1 h prior to 5 μg/ml LPS for 24 h in Nrf2 siRNA-transfected MLE12 cells

 NC siRNA + LPS + TMP

50 μg/ml TMP pre-incubated 1 h prior to 5 μg/ml LPS for 24 h in NC siRNA-transfected MLE12 cells

  1. Abbreviations: TMP Tetramethylpyrazine, LPS lipopolysaccharide, ALI acute lung injury, Nrf2 nuclear factor erythroid 2-related factor-2, HO-1 heme oxygenase-1