Fig. 5

AS-IV depressed pyroptosis and the production of fibrosis markers induced by hypoxia in PASMCs through the PHD2-HIF1α axis. A-D, PASMCs were divided into 5 groups: Nor, Hyp, AS, AS + si-PHD2 and LW6. Cropped blots of GSDMD-N, NLRP3, cleaved Caspase-1, Fibronectin and Collagen1 (the samples derived from the same experiment and that gels/blots were processed in parallel), and quantitation of the pyroptosis markers and the fibrosis markers. E, Cell death was detected by PI/Hoechst double fluorescent staining. Scale bar = 50 μm. F, IL-1β and IL-18 levels in cell supernatant were measured by ELISA. G, LDH activity was detected by a LDH release agent. H-I, Proteins of MMP2/9 and TIMP4 were detected by ELISA method. ^*P<0.05, ^**P<0.01, ^***P<0.001, comparison with Nor group. ^#P<0.05, ^##P<0.01, ^###P<0.001, comparison with Hyp group.^{^}P<0.05, ^{^^}P<0.01, ^{^^^}P<0.001, comparison with AS group