Fig. 2

Mechanical ventilation leads to diaphragm injuries and protein degradation. (A-C) TEM revealed ultrastructure damage to the diaphragms. Scale bar = 1 μm/500 nm. (D-E and G-H) Diaphragm protein lysates of the two groups were detected by Western blotting, and the expression of MAFbx and MuRF1 was quantified. (Before incubated with antibodies, the membranes were clipped according to target proteins molecular weight position on the instruction of the protein marker. Full-length blots are presented in Supplementary Fig. 3). (F and I) Diaphragms of the two groups were detected by qPCR to analyse MAFbx and MuRF1 expression. Values are represented as the mean ± SD. n = 6. (*p < 0.05, **p < 0.01, ***p < 0.001)