Fig. 3

Cellular senescence participates in ventilation-induced diaphragm dysfunction. (A) Volcano plot of differential gene expression of the CON group and the MV group (n = 3). (B) Heatmap of cellular senescence-related differential gene expression in the CON group and the MV group (n = 3). (C) Bubble chart of cellular senescence-related statistics of GO enrichment of the CON group and the MV group (n = 3). (D-G) The diaphragm of the two groups was subjected to qPCR to analyse Pai1, MMP9, CHEK2 and ANKRD1 expression (n = 6). (H-I) Diaphragmatic tissue sections and holistic pieces of diaphragms from the two groups were subjected to SA-βGal staining. Bar = 100 μm. Values are represented as the mean ± SD. (*p < 0.05, **p < 0.01)