Fig. 6

PEDF reduces LPS-induced apoptosis of RLE-6TN cells by upregulating PPAR-γ. A Western blotting to detect the expression of cleaved-caspase3 and rip3 to investigate the effect of the PPAR-γ inhibitor on PEDF treatment. B Quantification of the related protein expression. Data are expressed as the mean ± SD, n = 4. C Representative images of PPAR-γ immunofluorescence. Scale bars: 50 μm. D Quantification of the PPAR-γ fluorescence density. E Flow cytometry to detect the effect of the PPAR-γ inhibitor on PEDF treatment. F Quantification of the cell apoptosis. Data are expressed as the mean ± SD, n = 4, *P < 0.05, **P < 0.01 vs. LPS group or indicated group; #P < 0.05, ##P < 0.01 vs LPS + PEDF group