Fig. 2

KLK8 expression in LUSC tumor microarray (TMA). a KLK8 staining intensity scoring standard. KLK8 expression was graded into 4 categories: 0, 1, 2, and 3 according to the staining intensity. The larger number represented higher KLK8 staining intensity (Magnification, 100X. Scale bar, 50 μm). The final score of samples is evaluated by multiplying the grade and corresponding area percentages. b Example of high and low KLK8 expression in TMA. Left, high expression sample. Right, low expression sample (Magnification, 20X. Scale bar, 400 μm). c K-M curve showing the overall survival in LUSC TMA stratified by KLK8 median expression. Blue, KLK8 low expression group. Yellow, KLK8 high expression group. d Univariate analysis forest plot of clinicopathological factors, KLK8 expression, and other immune markers’ expression in LUSC TMA. CoExp, PD-L1, and CD47 co-expression. CD8+ TILs, CD8+ tumor infiltration lymphocytes. CD68+ M, CD68+ infiltration macrophage infiltration level. The red square represents factors that were statistically significant for prognosis, while the blue square represents factors that were without statistical significance for prognosis. e Multivariate analysis forest plot of factors, which could predict the overall survival in the univariate analysis. CoExp, PD-L1, and CD47 co-expression. CD8 + TILs, CD8+ tumor infiltration lymphocytes. The red square represents factors that were statistically significant for prognosis, while the blue square represents factors that were without statistical significance for prognosis