Fig. 4

VD3 inhibited inflammation and lung permeability disruption caused by hypoxic environment. Thirty SD rats were randomly divided into the normoxia group, hypoxia group, and vitamin D3 (VD3, 1,25-(OH)2-D3) group with 10 rats in each group. A and B Lung injury score assessed based on evaluation of H&E stain. C-E The concentrations of TNF-α, IL-6, and IL-1β in lung tissues were determined by ELISA. F–H The expression of occludin 4, VE-cadherin, and ZO-1 in lung tissues were evaluated by Western blot. β-actin served as a loading control. I and J IF stain for ZO-1 (magnification, × 400). Means and standard deviations (SD) were used to represent the data. Statistical analyses (two group comparisons) were performed using Students t-test. ^* P < 0.05 vs Normoxia, ^** P < 0.01 vs Normoxia, ^# P < 0.05 vs Hypoxia, ^## P < 0.01 vs Hypoxia