Fig. 5

VD3 suppressed Fga and Fgb expression in lung of hypoxia-induced rats. Thirty SD rats were randomly divided into the normoxia group, hypoxia group, and vitamin D3 (VD3, 1,25-(OH)2-D3) group with 10 rats in each group. A and B ELISA analysis of Fga and Fgb in lung tissues. C-E Expression of Fga and Fgb was detected by Western blot. Load control: β-actin. F and G IHC detection of Fga (magnification, × 400). H and I Fgb detection was performed by IHC stain (magnification, × 400). Arrows indicate cells that are positively expressed. Means and standard deviations (SD) were used to represent the data. Statistical analyses (two group comparisons) were performed using Students t-test. ^** P < 0.01 vs Normoxia, ^# P < 0.05 vs Hypoxia, ^## P < 0.01 vs Hypoxia