Fig. 6

VD3 weakened coagulation cascade in lung and liver of hypoxia-induced rats. Thirty SD rats were randomly divided into the normoxia group, hypoxia group, and vitamin D3 (VD3, 1,25-(OH)2-D3) group with 10 rats in each group. A and B The protein levels of TF, FVII, FII, FV, FX, and TM in lung and liver was tested by ELISA. C The contents of PAR1, PAR3, and PAR4 in lung was detected by ELISA. D and E The expression of PAR1, PAR3, and PAR4 in lung was analyzed by Western blot. Load control: β-actin. F and G The expression of C3, C3a, and C5 in lung were assayed by Western blot analysis. Load control: β-actin. H and I IHC detection of C3, C3a, and C5 in lung (magnification, × 400). Arrows indicate cells that are positively expressed. Means and standard deviations (SD) were used to represent the data. Statistical analyses (two group comparisons) were performed using Students t-test. ^** P < 0.01 vs Normoxia, ^# P < 0.05 vs Hypoxia, ^## P < 0.01 vs Hypoxia