Fig. 8

VD3 improved type II alveolar epithelial cell damage and inflammation induced by hypoxia. The cells were divided into four groups: normoxia group, hypoxia group, hypoxia + low-concentration VD3 group, and hypoxia + high-concentration VD3 group. A CCK-8 assay of type II alveolar epithelial cell proliferation. B LDH activity was determined using a commercial LDH assay kit. C Expression levels of cellular supernatant TNF-α, IL-6 and IL-1β were determined by ELISA. Means and standard deviations (SD) were used to represent the data. Statistical analyses (two group comparisons) were performed using Students t-test. A one-way ANOVA with Tukey post hoc test of means was used for multiple group comparisons. ^** P < 0.01 vs Normoxia, ^# P < 0.05 vs Hypoxia, ^## P < 0.01 vs Hypoxia