Fig. 9

VD3 inhibited complement and coagulation cascade in hypoxia-induced co-culture system of alveolar epithelial cells and hepatocytes. A and B Expression of Fga and Fgb was detected by Western blot. Load control: β-actin. C The protein levels of TF, FVII, FII, FV, FX, and TM in type II alveolar epithelial cells were tested by ELISA. D-G The expression of PAR1, PAR3, PAR4, C3, C3a, and C5 in type II alveolar epithelial cells was analyzed by Western blot. Load control: β-actin. Means and standard deviations (SD) were used to represent the data. Statistical analyses (two group comparisons) were performed using Students t-test. A one-way ANOVA with Tukey post hoc test of means was used for multiple group comparisons. ^** P < 0.01 vs Normoxia, ^# P < 0.05 vs Hypoxia, ^## P < 0.01 vs Hypoxia