Fig. 2

Alveolar macrophage depletion decreased the gVPLA2 content in BALF, downregulated intrapulmonary p-cPLA2/PGE2 and decreased intracellular Ca2+. A The gVPLA2 levels in BALF. B Relative immunofluorescence mean density of p-cPLA2 in the lung tissue of each group of mice. C Immunohistochemical scores of p-cPLA2 in the lung tissue of each group of mice. D Levels of PGE2 in lung tissue. E Immunohistochemical detection of intrapulmonary p-cPLA2 expression; magnification, 400× (indicated by the red arrow). F Immunofluorescence detection of intracellular p-cPLA2 expression in lung, magnification, 400×. G Intracellular calcium ion content in single cell suspensions of lung tissue by Fluo-4AM, magnification, 200×. Data are shown as the mean ± SD (n = 6). aP < 0.05 vs. CON and NTV group, bP < 0.05 vs. HTV group. CON, blank control; NTV, normal tidal volume; HTV, high tidal volume; CLOD, macrophage-depletion combined with high tidal volume