Fig. 3

Inhibition of alveolar macrophage gVPLA2 decreased the level of gVPLA2 in alveolar lavage fluid, downregulated intracellular cPLA2/PGE2 and reduced intracellular Ca2+. A Levels of gVPLA2 in BALF. B Immunohistochemical scores of p-cPLA2 in lung tissue. C Immunohistochemical detection of intracellular cPLA2 expression in lungs, magnification, 400× (indicated by the red arrow). D Immunofluorescence detection of intracellular p-cPLA2 expression in lung, magnification, 400×. E Relative immunofluorescence mean density of p-cPLA2 in lung tissue. F Levels of PGE2 in lung tissue. G Intracellular calcium ion content in single cell suspensions of lung tissue by Fluo-4AM, magnification, 200×. Data are shown the mean ± SD (n = 6). aP < 0.05 vs. CON and NTV group, bP < 0.05 vs. HTV group, cP < 0.05 vs. BMDM group. CON, blank control; NTV, normal tidal volume; HTV, large tidal volume; CLOD, macrophage-depletion combined with high tidal volume; BMDM, alveolar macrophage depletion, followed by intratracheal drip of normal bone marrow-derived macrophages in the high tidal volum; VAR, alveolar macrophage depletion, followed by intratracheal drip of Group V phospholipase A2 inhibitor pretreated bone marrow-derived macrophages in the high tidal volume