Fig. 4

Inhibition of alveolar macrophage gVPLA2 ameliorates mitochondrial kinetic disorders and mitochondrial dysfunction. A Transmission electron microscopic observation of structural and morphological changes of mitochondria in alveolar epithelial cells, scale bar, 2 μm(top), 500 nm(bottom). B Protein immunoblotting to detect the expression of the mitochondrial splitting protein DRP1 and the mitochondrial fusion proteins OPA1 and MFN2. C-E The relative expression of DRP1, OPA1 and MFN2 in the lungs of each group of mice using GAPDH as the internal reference protein. F The levels of ATP. Data are shown as the mean ± SD (n = 6). aP < 0.05 vs. CON and NTV group, bP < 0.05 vs. HTV group, cP < 0.05 vs. BMDM group. CON, blank control; NTV, normal tidal volume; HTV, large tidal volume; CLOD, macrophage-depletion combined with high tidal volume; BMDM, alveolar macrophage depletion, followed by intratracheal drip of normal bone marrow-derived macrophages in the high tidal volum; VAR, alveolar macrophage depletion, followed by intratracheal drip of Group V phospholipase A2 inhibitor pretreated bone marrow-derived macrophages in the high tidal volume