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Fig. 5 | BMC Pulmonary Medicine

Fig. 5

From: Alveolar macrophage-derived gVPLA2 promotes ventilator-induced lung injury via the cPLA2/PGE2 pathway

Fig. 5

Human recombinant gVPLA2 upregulates p-cPLA2/PGE2 in A549 cells and increases intracellular Ca2+. A Relative immunofluorescence indicates the density of intracellular p-cPLA2 in different groups. B Immunofluorescence detection of intracellular p-cPLA2 expression in A549 cells, magnification, 400×. C Intracellular PGE2 levels in different groups. D Detection of cytoplasmic calcium ions in A549 cells by Fluo-4 AM (green light) and intracellular mitochondrial calcium content in A549 cells by Rhod-2 AM (red light), magnification, 400×. Data are shown the mean ± SD (n = 6). dP < 0.05 vs. CON and DMSO groups. CON, blank control; DMSO, dimethyl sulfoxide solvent control; PLA, human recombinant gVPLA2-treated

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BMC Pulmonary Medicine

ISSN: 1471-2466

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