Fig. 7

Inhibition of alveolar macrophage gVPLA2 attenuates lung injury and the inflammatory response. A HE staining for lung histopathological injury; magnification, 200×. B Pathology score. C The wet/dry ratio of lung tissue. D Total cell count in BALF. E Total protein content in BALF. F TNF-α level in BALF. G IL-6 level in BALF. Data are shown as the mean ± SD (n = 6). aP < 0.05 vs. CON and NTV group, bP < 0.05 vs. HTV group, cP < 0.05 vs. BMDM group. CON, blank control; NTV, normal tidal volume; HTV, large tidal volume; CLOD, macrophage-depletion combined with high tidal volume; BMDM, alveolar macrophage depletion, followed by intratracheal drip of normal bone marrow-derived macrophages in the high tidal volum; VAR, alveolar macrophage depletion, followed by intratracheal drip of Group V phospholipase A2 inhibitor pretreated bone marrow-derived macrophages in the high tidal volume