Fig. 1

The hypoxia-induced PH mouse model was successfully established and the expression of p-JAK2 and p-STAT3 were increased, while the expression of SOCS5 was reduced in PH mice. A&B Increased RVSP and RVHI in lung sections from mice exposed to normoxia (Control) or hypoxia (PH) for 4 weeks (n = 6 each group). C Representative photographs were shown of H&E staining of pulmonary arteries from control and PH mice. Scale bar = 100 μm. The percentage of the inner wall thickness was quantified (n = 6 each group). D Representative images of IHC of pulmonary arteries from control and PH mice to detect p-JAK2 and p-STAT3. Scale bar = 50 μm. E SOCS1, SOCS2, SOCS3 and SOCS5 mRNA expression of whole lungs from control and PH mice (n = 6 each group). F Western blot analysis and quantification of SOCS5 in whole lungs of Control and PH mice (n = 6 each group). G Representative photographs of immunofluorescence staining for SOCS5 and α-SMA in whole lungs of control and PH mice. Scale bar = 50 μm. PH, pulmonary hypertension; RVSP, right ventricular systolic pressure; RVHI, right ventricular hypertrophy index; H&E, hematoxylin–eosin; IHC, immunohistochemistry