Fig. 2

SOCS5 expression in hypoxia-induced HPASMCs. A HPASMCs were identified by immunofluorescence staining for α-SMA under normoxia. Scale bar = 50 μm. B Time-dependent changes in SOCS5 mRNA expression following HPASMCs under hypoxia (n = 3 each group). C Western blot analysis and quantification of SOCS5 protein expression in HPASMCs induced by hypoxia for 0, 6, 24 and 72 h. HPASMCs, human pulmonary arterial smooth muscle cells