Fig. 3

Effects of SOCS5 expression on HPASMC proliferation and migration under hypoxia conditions. A-C After HPASMCs were transfected with plasmids overexpressing or knocking down SOCS5 for 24 h, starved for 3 h and then treated with normoxia or hypoxia for 72 h, MTT was used to verify cell viability (A) (n = 3 each group). Proliferation of HPASMCs was evaluated by EdU incorporation assay and EdU-positive cells were quantified (B) (n = 3 each group). Scale bar = 50 μm. Migration of HPASMCs was assayed by transwell assay (C) (n = 3 each group). Scale bar = 100 μm. HPASMCs, human pulmonary arterial smooth muscle cells; RT-qPCR, quantitative reverse transcription polymerase chain reaction; MTT, 3- (4,5)-dimethylthiahiazo (-z-y1)-2,5-di- phenytetrazoliumromide; EdU, 5-Ethynyl-2’- deoxyuridine