Fig. 5

SOCS5 knockdown activated the JAK2/STAT3 signaling in HPASMCs. A Western blot analysis and quantification of JAK2, p-JAK2^Tyr1007/1008, STAT3 and p-STAT3^Tyr705 protein expression in HPASMCs, which were transfected with plasmids overexpressing or knocking down SOCS5 for 24 h, starved for 3 h and then treated with normoxia or hypoxia for 72 h. B-E After HPASMCs were transfected with plasmids knocking down SOCS5 for 24 h, starved for 3 h, supplemented with AG490 (10 μM) and then treated with hypoxia for 72 h, western blot analysis and quantification of STAT3 and p-STAT3^Tyr705 protein expression were performed (B) (n = 3 each group). MTT was used to verify cell viability (C) (n = 3 each group). Migration of HPASMCs was assayed by transwell assay (D) (n = 3 each group). Representative image of cell contraction assay and quantification of collagen contraction relative to the initial collagen gel area was performed (E) (n = 3 each group). HPASMCs, human pulmonary arterial smooth muscle cells; MTT, 3- (4,5)-dimethylthiahiazo (-z-y1)-2,5-di- phenytetrazoliumromide