Fig. 6

SOCS5 overexpression inhibited the JAK2/STAT3 signaling in HPASMCs. (A-D) After HPASMCs were transfected with plasmids overexpressing SOCS5 for 24 h, starved for 3 h, supplemented with Butyzamide and then treated with hypoxia for 72 h, western blot analysis and quantification of STAT3 and p-STAT3^Tyr705 protein expression were performed (A) (n = 3 each group). MTT was used to verify cell viability (B) (n = 3 each group). Migration of HPASMCs was assayed by transwell assay (C) (n = 3 each group). Representative image of cell contraction assay and quantification of collagen contraction relative to the initial collagen gel area was performed (D) (n = 3 each group). HPASMCs, human pulmonary arterial smooth muscle cells; MTT, 3- (4,5)-dimethylthiahiazo (-z-y1)-2,5-di- phenytetrazoliumromide