Fig. 7

SOCS5 was regulated by hsa-miR-155-5p. A RT-qPCR was performed to detect hsa-miR-155-5p mRNA expression in HPASMCs cultured under normoxia or hypoxia conditions (n = 3 each group). B Prediction of the relationship between hsa-miR-155-5p and SOCS5. C Luciferase activity of 293 T cells transfected with plasmids carrying a wild-type or mutant 3′UTR of SOCS5 in response to hsa-miR-155-5p mimic. D-E RT-qPCR and western blot analysis of the expression of SOCS5 in HPASMCs transfected with plasmids carrying hsa-miR-155-5p mimic or inhibitor (n = 3 each group). F-I After HPASMCs were transfected with plasmids carrying hsa-miR-155-5p inhibitor and knocking down SOCS5 for 24 h, starved for 3 h and then treated with hypoxia for 72 h, MTT was used to verify cell viability (F) (n = 3 each group). Migration of HPASMCs was assayed by transwell assay (G&H) (n = 3 each group). Representative image of cell contraction assay and quantification of collagen contraction relative to the initial collagen gel area was performed (I) (n = 3 each group). RT-qPCR, quantitative reverse transcription polymerase chain reaction; HPASMCs, human pulmonary arterial smooth muscle cells; MTT, 3- (4,5)-dimethylthiahiazo (-z-y1)-2,5-di- phenytetrazoliumromide