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Fig. 3 | BMC Pulmonary Medicine

Fig. 3

From: Unveiling the potent effect of vitamin D: harnessing Nrf2/HO-1 signaling pathways as molecular targets to alleviate urban particulate matter-induced asthma inflammation

Fig. 3

Vitamin D activates the Nrf2/HO-1 signaling pathway in mouse asthma. (a) qPCR was used to analyze Nrf2, HO-1 and NGF’s gene expression levels in lung tissue. (b) immunoblot analysis of the protein expression of Nrf2 and HO-1 in lung tissue. (c) gray-scale values count the relative protein expression of Nrf2 (nucleus) relative to Nrf2 (cytoplasm) and HO-1 relative to β-actin. (d) qPCR analysis of iNOS gene expression level in lung tissue. (e) immunoblot analysis of the protein expression of iNOS in lung tissue. (f) gray-scale values count the relative protein expression of iNOS relative to β-actin. (g) ELISA analysis of the NGF protein expression levels in BALF and serum. (* p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001)

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BMC Pulmonary Medicine

ISSN: 1471-2466

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