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Fig. 4 | BMC Pulmonary Medicine

Fig. 4

From: Unveiling the potent effect of vitamin D: harnessing Nrf2/HO-1 signaling pathways as molecular targets to alleviate urban particulate matter-induced asthma inflammation

Fig. 4

Vitamin D reversed Nrf2/HO-1’s low expression levels in ASMCs induced by PM or in ASMCs treated with HO-1 shRNA lentiviral vector. (a) qPCR was used to analyze Nrf2/HO-1 and NGF’s gene expression levels. (b) immunoblot analysis of the protein expression levels of Nrf2 and HO-1 on ASMCs treated using different substances. (c) gray-scale values count the relative protein expression of Nrf2 (nucleus) relative to Nrf2 (cytoplasm) and HO-1 relative to β-actin in ASMCs. (d) After treating ASMCs with different substances, ELISA was used to analyze NGF’s protein expression level in cell supernatants. (e) The Lenti-sh-HO-1 stable cell line. (* p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001)

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BMC Pulmonary Medicine

ISSN: 1471-2466

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