Fig. 4

IRF4 knockdown attenuates the conversion of FOXP3⁺ Tregs to FOXP3⁺RORγt⁺ Tregs in the lung tissues of mice after hyperoxia induction. a Schematic diagram of the construction of IRF4-KO mice by CRISPR/Cas9 technology. b Deletion of IRF4 was confirmed by Western blotting (n = 5). c Flow cytometry analysis of FOXP3⁺ Tregs in the mouse lung tissues of WT-hyperoxia and KO-hyperoxia groups. d Flow cytometry analysis of FOXP3⁺RORγt⁺ Tregs in the mouse lung tissues of the WT-hyperoxia and KO-hyperoxia groups. The representative data from five independent experiments. Data are presented as mean ± SD (n = 5, t-test one-way); *P < 0.05 and **P < 0.01 vs. WT-hyperoxia group