Table 2 Hole types used for validation experiment
Blank hole | Standard hole | Measuring hole | |
|---|---|---|---|
Deionized water | 10 µL | ||
1mmol/L Calcium standard solution (1 mmol/L) | 10 µL | ||
Tissue-homogenate supernatant | 10 µL | ||
Working fluid II | 250 µL | 250 µL | 250 µL |
After mixing well and leaving for 5 min, the absorbance of each hole was measured by colorimetry at 610 nm | |||