Fig. 6

Photomicrographs of intracellular mycobacteria detected by double staining. a Macrophages (*), b Neutrophils (□), c Pneumocytes-II (∆).Slides stained by combination of auramine-rhodamine (for mycobacteria) and IHC of CD 68 (macrophages), NE (neutrophils) and SP-C (pneumocytes-II), respectively. The fluorescence signals of mycobacteria are red. Brightfield was used to capture immunohistochemical detections. Images were color-inversed to negative form which is why brown color of the color-substrate diaminobenzidine was shifted to blue. Overlays of the fluorescent and negative images were performed by using FixFoto. All images were acquired at × 400 magnification, scale bar =100 μm