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Effects of periodontal treatment on exacerbation frequency and lung function in patients with chronic periodontitis: study protocol of a 1-year randomized controlled trial
BMC Pulmonary Medicine volume 17, Article number: 23 (2017)
- The Erratum to this article has been published in BMC Pulmonary Medicine 2017 17:51
Chronic obstructive pulmonary disease (COPD) has been associated with periodontal disease (PD), and periodontal treatment (PT) has been connected to reduction of lung disease exacerbations. Bronchiectasis has many clinical similarities with COPD but, although it is also a chronic lung disease, to date it has not been studied with relation to PD. The aim of this study is to evaluate whether PT associated with photodynamic therapy (PDT) reduces the number of exacerbations, improves pulmonary function, periodontal clinical parameters and quality of life after 1 year of periodontal treatment follow-up.
Bronchiectasis patients will undergo medical anamnesis and periodontal examination. Participants with periodontitis will be divided into two groups and PT will be performed as G1 control group (n = 32) – OHO (oral hygiene orientation) + supragingival treatment + simulation of using photodynamic therapy (PDT); G2 experimental (n = 32) – scaling and root planing + PDT + OHO. Lung function will be assessed both at baseline and after 1 year by spirometry, exacerbation history will be analyzed through clinical records monitoring. Three instruments for quality of life assessment will also be applied – Saint George’s Respiratory Questionnaire and Impact Profile Analysis Oral health (OHIP-14). It is expected that periodontal treatment can improve the analyzed parameters after 1 year.
Although only one study evaluates exacerbation in COPD after 1 year of PT, bronchiectasis has not been studied in the dentistry field to date. Trial registration: NCT02514226. Version #1. This study protocol receives grant from FAPESP (São Paulo Research Foundation) #2015/20535-1. First received: July 22, 2015, 1st version. This protocol has been approved by the Research Ethics Committee of Nove de Julho University.
In recent years, many studies have associated periodontal disease (PD) and pulmonary diseases ; they have also connected decreased lung function to loss of periodontal attachment . Chronic obstructive pulmonary disease (COPD) is the most studied lung disease related to PD. The pathophysiological process linking both conditions is the destruction of connective tissue and exacerbated inflammatory reaction with large recruitment of neutrophils . In 1 year, periodontal treatment (PT) was able to reduce annual number of COPD exacerbations from 9 to 4 .
Bronchiectasis is also a chronic, irreversible lung disease characterized by permanent bronchi dilation. However, it has not yet been studied in the dental field. It is a condition that causes high morbidity, affecting patients’ quality of life . In Brazil, its prevalence is estimated at 4–18% . Many health professionals are not familiar with its diagnosis and, hence, it is often diagnosed as asthma or COPD due to similarities in clinical manifestations , such as cough and sputum production . Bronchiectasis patients suffer with recurrent acute exacerbations, which sometimes require hospitalization, increasing public health expending. These patients exacerbate an average of 6.6 times per year  and clinical treatment is based on prevention and control of exacerbation episodes, leading to decreased number of hospitalizations.
The etiology of bronchiectasis is extremely heterogeneous and may involve genetic as well as acquired causes. The pathophysiology of bronchiectasis development involves mucociliary changes that result in bacterial colonization and subsequent chronic inflammation with damage to the bronchi . Periodontal pathogens have been found in infected lung fluid [10–12]. Thus, there is biological plausibility in the assertion that a decrease in oral bacterial load could reduce the number of pulmonary exacerbations [5, 6]. To confirm this data, it is essential to measure PT contribution in improving bronchiectasis patients’ quality of life.
Hence, clinical randomized controlled trials are necessary to test if the reduction in oral infection decreases the number of exacerbations . So, the hypothesis of this study is that periodontal treatment can reduce episodes of bronchiectasis exacerbation and improves bronchiectasis patients’ quality of life after 1 year. Therefore, the aim of this study is to determine if periodontal treatment leads to fewer episodes of bronchiectasis exacerbation and improves bronchiectasis patients’ quality of life after 1 year.
A randomized, controlled, 12-month, parallel-group clinical trial, registered at www.clinicaltrial.gov (NCT02514226) will be performed. After verbal and written explanation of the study, patients will sign the Informed Consent Form approved by the Research Ethics Committee (# 1.057.901) Universidade Nove de Julho (UNINOVE). A researcher not involved in the study will enroll patients. Forty patients under medical care at InCor-FMUSP with generalized chronic periodontitis will receive periodontal treatment at Universidade Nove de Julho’s Dental Clinic, from August 2016 to September 2018.
Sample size calculation
For this study, based on exacerbations of bronchiectasis, we assume that the difference between the groups will be one exacerbation with a standard deviation of 1.4  with an effect size of 1. Thirty-two (32) patients per group will be necessary with a significance level of 5%, a power of 80% in a two-group t-test, using the statistical package Dell Statistica version 12.
Inclusion and exclusion criteria
Patients ≥35 years, of both genders, clinically stable with more than 10 teeth and chronic moderate periodontal disease, i.e., more than 15% of examined teeth with ≥4 mm probing depth (PD) will be included . Patients will be included if they have bronchiectasis, characterized clinically by chronic sputum production and dilated bronchi, and confirmed by chest computed tomography scan. Severity of bronchiectasis will be assessed according to FACED score .
Smokers or former smokers who have quit for less than 5 years will be excluded. Also, pregnant women, patients with other lung diseases, such as cystic fibrosis, COPD and asthma, those who are taking medications that affect periodontal condition (e.g., phenytoin or cyclosporine), patients with decompensated systemic diseases, those who required prophylactic antibiotic therapy before periodontal treatment or those who have undergone periodontal treatment less than 6 months prior will be excluded.
Anamnesis and physical examination
In the anamnesis, questions will be asked about the patients’ general health. Demographic data (age, gender, marital status, occupation, educational level, living conditions and salary) will be collected, as well as medical history data (main complaint, current state of illness, medical and dental history, and medication use), habits and addictions (smoking, drinking, nutrition and oral hygiene).
On physical examination, clinical periodontal parameters will be collected by a single researcher. The calibration will be performed by a single researcher at six sites per tooth for probing depth (PD) and clinical attachment level (CAL) according to Pinto et al. (2016) . The data will undergo the intraclass correlation coefficient test – ICC . Agreement with standard examiner must be >80%.
This researcher will not be involved in the periodontal treatment of bronchiectasis patients. The following periodontal parameters will be evaluated: probing depth (PD), clinical attachment level (CAL), gingival index (GI), visible plaque index, probing bleeding (SS) and suppuration (S) (Ainamo, Bay 1975) .
Three questionnaires will be used to assess bronchiectasis patients’ quality of life: Saint George’s Respiratory Questionnaire – SGRQ, CoLB, and OHIP-14. They will be applied at baseline and after 12 months after periodontal treatment (Fig. 1).
Analysis of Oral Health Impact Profile (OHIP-14 questionnaire)
The Oral Health Impact Profile (OHIP-14) is a simplified form of the original questionnaire OHIP used for assessment of oral health impact on quality of life . Items are distributed among the following subscales: functional limitation, pain, psychological discomfort, physical disability, psychological disability, social disability, and deficit.
Saint George’s Respiratory Questionnaire – SGRQ
The specific Saint George’s Respiratory Questionnaire (SGRQ) is used to assess quality of life in patients with pulmonary disease . Responses will be evaluated by two experienced physiotherapists (S.D.C. and F.D.C.) that will be calibrated and blinded to perform the evaluation.
Spirometry tests will be performed by two physiotherapists (S.D.C. and F.D.C.) using ULTIMA CPX (MedGraphics Corporation®, St. Paul, MN, USA) with a calibrated pneumotachograph. The technical procedures, acceptance criteria and reproducibility are recommended by the Brazilian Consensus on Spirometry . All patients will perform this procedure after using a bronchodilator (salbutamol 400 μcg inhalation). Variables recorded include FVC (forced vital capacity), FEV1 (forced expiratory volume in one second timed) and FEV1/FVC. The values will be expressed in absolute values and percentage of the predicted for the Brazilian population .
Randomization will be performed as described by Pinto et al. (2016)  using Microsoft Excel, version 2013. Forty patients will be randomly distributed into experimental and control groups. The randomization will be blocked in groups of four patients.
Opaque envelopes will be identified with sequential numbers and inside of each of them a sheet will be inserted containing the corresponding group information, according to the random order obtained. The envelopes will be sealed and remain sealed in numerical order in a safe place until the time of periodontal treatments.
Patients will be allocated into experimental and control groups as follows:
Group 1 – control group (n = 32) – OHO (oral hygiene orientation) + supragingival treatment + simulation of using photodynamic therapy (PDT)
Group 2 – experimental (n = 32) – scaling and root planning + PDT + OHO (Table 1).
Photodynamic therapy (PDT)
Patients of Group 2 will receive photodynamic therapy. PDT associates a photosensitizer with light source in order to reduce the microbial load. The procedures will be performed as described.
Scaling and root planning
Application of methylene blue (0.005% - Chimiolux 10, DMC® – Purified water and methylene blue) with a carpule syringe and needle (with stop and without bevel) into the periodontal pockets >4 mm
Wait for 3 min (Betsy )
Irradiation will be performed with red laser diode (λ = 660 nm) with an output power of 100 mW (MM Optics Twin Laser, São Paulo, SP, Brazil) in all sites around a tooth (six sites per tooth) on all teeth, lastly, 1 min irradiation scanning around each tooth.
Washing abundantly with saline until complete removal of methylene blue
Every adverse effects will be related.
Patients of control group will receive simulation of using photodynamic therapy (PDT).
The simulation of PDT will be performed by applying methylene blue (0.005% - Chimiolux 10, DMC® – Purified water and methylene blue) with a carpule syringe and needle (with stop and without bevel) into the periodontal pockets >4 mm. Then, wait for 3 min (Betsy ). Irradiation will be performed with the laser (MM Optics Twin Laser, São Paulo, SP, Brazil) turned off in all sites around a tooth (six sites per tooth) on all teeth, lastly, 1 min irradiation scanning around each tooth. Than, the operator will wash abundantly with saline until complete removal of methylene blue.
The Liliefors test will assess the normality of the data. If the data are normal, t test (Bioestat 5.3, Pará, Brazil) will be used to compare continuous and dependent variables of Groups 1 and 2 (periodontal parameters). If the data do not have normal distribution, Mann Whitney test will be used. Baseline data will be compared with data from 12 months after treatment. The value of p <0.05 will be considered statistically significant. The x 2 test will be used to compare categorical variables between Groups 1 and 2. All values will be expressed as mean ± Standard Deviation.
The primary variable of the study will be the number of exacerbations. Secondary variables will include spirometry (lung evaluation), dental clinical parameters (periodontal examination), and quality of life parameters (SGRQ, and OHIP-14 questionnaires). These variables will be assessed at baseline and 12 months after periodontal treatment.
Strategies for achieving adequate participant enrolment to reach target sample size
Patients will be evaluated every 3 months in order to motivate and hygiene control.
Lung diseases, such as chronic obstructive pulmonary disease (COPD) and bronchiectasis, cause impairment to a patient’s quality of life with large numbers of comorbidities. It has been shown that periodontal treatment (PT) and oral hygiene (OH) deceases the severity of COPD ; but, despite clinical and pathophysiological similarities between COPD and bronchiectasis , there is no study to date about the relationship of bronchiectasis with periodontal disease (PD). Patients with bronchiectasis have multiple episodes of disease exacerbations. Interventions, such as physical exercises and chronic administration of certain drugs decrease the number of such occurrences, but no intervention can achieve results within less than 6 months [21–30]. In this study, we will perform an oral intervention with a 1-year follow up. We will measure the number of exacerbations, spirometry data, dental clinical parameters and quality of life parameters (SGRQ, and OHIP-14 questionnaires). Saint George’s Respiratory Questionnaire is the most complete instrument, considered the gold standard for lung diseases, and OHIP-14, which analyze the impact of oral health profile. It is expected that periodontal treatment maintained for 1 year will help reduce the number of exacerbations, improve lung mechanics and quality of life in bronchiectasis patients.
Chronic obstructive pulmonary disease
forced expiratory volume in one second timed
Forced vital capacity
Saint George’s Respiratory Questionnaire
Agado BE, Crawford B, DeLaRosa J, Bowen DM, Peterson T, Neill K, Paarmann C. Effects of periodontal instrumentation on quality of life and illness in patients with chronic obstructive pulmonary disease: a pilot study. J Dent Hyg. 2012;86:204–14.
Zeng XT, Tu ML, Liu DY, Zheng D, Zhang J, Leng W. Periodontal disease and risk of chronic obstructive pulmonary disease: a meta-analysis of observational studies. PLoS One. 2012;7:e46508.
Usher AK, Stockley RA. The link between chronic periodontitis and COPD: a common role for the neutrophil? BMC Med. 2013 Nov 13;11:241.
Zhou X, Han J, Liu Z, Song Y, Wang Z, Sun Z. Effects of periodontal treatment on lung function and exacerbation frequency in patients with chronic obstructive pulmonary disease and chronic periodontitis: a 2-year pilot randomized controlled trial. J Clin Periodontol. 2014;41:564–72.
Ramos-Cerqueira AT, Crepaldi AL. Qualidade de vida em doenças pulmonares crônicas: aspectos conceituais e metodológicos. J Pneumol. 2000;26:207–13.
Athanazio RA, et al. Deve-se extrapolar o tratamento de bronquiectasias em pacientes com fibrose cística para aqueles com bronquiectasias de outras etiologias? J Bras Pneumol. 2010;36:425–31.
Sociedade Brasileira de Pneumologia e Tisiologia. Diretrizes para testes de função pulmonar. J Pneumol. 2002;28:S1–S82.
Pinto EH, Longo PL, de Camargo CC, Dal Corso S, Lanza Fde C, Stelmach R, Athanazio R, Fernandes KP, Mayer MP, Bussadori SK, Mesquita Ferrari RA, Horliana AC. Assessment of the quantity of microorganisms associated with bronchiectasis in saliva, sputum and nasal lavage after periodontal treatment: a study protocol of a randomised controlled trial. BMJ Open. 2016;6:e010564.
Bansal M, Khatri M, Taneja V. Potential role of periodontal infection in respiratory diseases - a review. J Med Life. 2013;6:244–8.
Ledić K, Marinković S, Puhar I, Spalj S, Popović-Grle S, Ivić-Kardum M, Samarzija M, Plancak D. Periodontal disease increases risk for chronic obstructive pulmonary disease. Coll Antropol. 2013;37:937–42.
Travis J, Pike R, Imamura T, Potempa J. The role of proteolytic enzymes in the development of pulmonary emphysema and periodontal disease. Am J Respir Crit Care Med. 1994;150:S143–6.
King PT, Holmes PW. Use of antibiotics in bronchiectasis. Rev Recent Clin Trials. 2012;7:24–30.
Tan L, Wang H, Li C, Pan Y.16S rDNA-based metagenomic analysis of dental plaque and lung bacteria in patients with severe acute exacerbations of chronic obstructive pulmonary disease. J Periodontal Res. 2014 Dec;49(6):760-9Apr 6.
Martínez-García MÁ, de Gracia J, Vendrell Relat M, et al. Multidimensional approach to non-cystic fibrosis bronchiectasis: the FACED score. Eur Respir J. 2014;43:1357–67.
Drain M, Elborn JS, Bergin DA, Hurley K, Mehta A, Cox S, Ryan D, O’Neill SJ, Reeves EP, McElvaney NG. J Inflamm Res. 2013;6:1–11.
Ainamo J, Bay I. Problems and proposals for recording gingivitis and plaque. Int Dent J. 1975;25:229–35.
Eltas A, Uslu MO, Eltas SD.Association of Oral Health-related Quality of Life with Periodontal Status and Treatment Needs.Oral Health Prev Dent. 2016;14(4):339-47. doi:10.3290/j.ohpd.a35613.
Souza TC, Jardim JR, Jones P. Validação do questionário do hospital Saint George na doença respiratória (SGRQ) em pacientes portadores de doença pulmonar obstrutiva crônica no Brasil. J Pneumol. 2000;26:119–25.
Betsy J, Prasanth CS, Baiju KV, Prasanthila J, Subhash N. Efficacy of antimicrobial photodynamic therapy in the management of chronic periodontitis: a randomized controlled clinical trial. J Clin Periodontol. 2014;41:573–81.
Gale NS, Bolton CE, Duckers JM, Enright S, Cockcroft JR, Shale DJ. Systemic comorbidities in bronchiectasis. Chron Respir Dis. 2012;9(4):231-8.
Lee AL, Hill CJ, Cecins N, Jenkins S, McDonald CF, Burge AT, Rautela L, Stirling RG, Thompson PJ, Holland AE. The short and long term effects of exercise training in non-cystic fibrosis bronchiectasis--a randomised controlled trial. Respir Res. 2014;15:44.
Gan WQ, Man SF, Senthilselvan A, Sin DD. Association between chronic obstructive pulmonary disease and systemic inflammation: a systematic review and a meta-analysis. Thorax. 2004;59:574–80.
Khoo JK, Venning V, Wong C, Jayaram L. J Clin Med. 2016;5: E115.Bronchiectasis in the Last Five Years: New Developments.
Karadak B, Karakoc F, Ersu R, Kut A, Bakac S, Dagli E. Non-cystic-fibrosis bronchiectasis in children: a persisting problem in developing countries. Respiration. 2005;72:233–8.
Zijlstra EE, Swart GR, Godfroy FJ, Degener JE. Pericarditis, pneumonia and brain abscess due to a combined Actinomyces--Actinobacillus actinomycetemcomitans infection. J Infect. 1992;25:83–7.
Rams TE, Degener JE, van Winkelhoff AJ. Antibiotic resistance in human chronic periodontitis microbiota. J Periodontol. 2014;85:160–9.
Albandar JM, Kingman A. Gingival recession, gingival bleeding, and dental calculus in adults 30 years of age and older in the United States, 1988–1994. J Periodontol. 1999;70:30–43.
Oliveira BH, Nadanovsky P. Psychometric properties of the Brazilian version of the Oral Health Impact Profile-short form. Community Dent Oral Epidemiol. 2005;33:307–14.
Wilson CB, Jones PW, O’leary CJ, Cole PJ, Wilson R. Validation of the St. George’s respiratory questionnaire in bronchiectasis. Am J Respir Crit Care Med. 1997;156:536–41.
Cole PJ. Inflammation: a two-edged sword--the model of bronchiectasis. Eur J Respir Dis Suppl. 1986;147:6–15.
This study protocol receives grant from FAPESP (São Paulo Research Foundation) #2015/20535-1.
This study protocol receives grant from FAPESP (São Paulo Research Foundation) grant #2015/20535-1. http://www.bv.fapesp.br/pt/pesquisador/687758/anna-carolina-ratto-tempestini-horliana.
Availability of data and material
Will be presented within the manuscript and/or additional supporting files.
SRS - has made substantial contributions to conception and design, or acquisition of data, and analysis and interpretation of data; EHP- has made substantial contributions to conception and design, or acquisition of data, and analysis and interpretation of data; PLL - has made substantial contributions to conception and design, or acquisition of data, and analysis and interpretation of data; SDC - has been involved in drafting the manuscript or revising it critically for important intellectual content; FCL- has been involved in drafting the manuscript or revising it critically for important intellectual content; RS, has given final approval of the version to be published, SZR- has been involved in drafting the manuscript or revising it critically for important intellectual content; ALdS- has been involved in drafting the manuscript or revising it critically for important intellectual content; MPAM- has given final approval of the version to be published; SKB- has been involved in drafting the manuscript or revising it critically for important intellectual content; KPSF-has been involved in drafting the manuscript or revising it critically for important intellectual content; RAMF- has been involved in drafting the manuscript or revising it critically for important intellectual content; ACRTH - has given final approval of the version to be published. All authors read and approved the final manuscript.
The authors declare that they have no competing interests.
Consent for publication
Ethics approval and consent to participate
The Research Ethics Committee of Nove de Julho University has approved this protocol (#1.057.901). All patients will sign the Informed Consent Form (# 1.057.901) also approved by the Research Ethics Committee Universidade Nove de Julho.
An erratum to this article is available at http://dx.doi.org/10.1186/s12890-017-0394-6.
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Cite this article
Santos, S.R., Pinto, E.H., Longo, P.L. et al. Effects of periodontal treatment on exacerbation frequency and lung function in patients with chronic periodontitis: study protocol of a 1-year randomized controlled trial. BMC Pulm Med 17, 23 (2017). https://doi.org/10.1186/s12890-016-0340-z
- Respiratory medicine
- Periodontal treatment
- Periodontal disease