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Design for a multicenter, randomized, sham-controlled study to evaluate safety and efficacy after treatment with the Nuvaira® lung denervation system in subjects with chronic obstructive pulmonary disease (AIRFLOW-3)



Targeted lung denervation (TLD) is a bronchoscopically delivered ablation therapy that selectively interrupts pulmonary parasympathetic nerve signaling. The procedure has the potential to alter airway smooth muscle tone and reactivity, decrease mucous secretion, and reduce airway inflammation and reflex airway hyperresponsiveness. Secondary outcome analysis of a previous randomized, sham-controlled trial showed a reduction in moderate-to-severe exacerbations in patients with COPD after TLD treatment. A pivotal trial, AIRFLOW-3 has been designed to evaluate the safety and efficacy of TLD combined with optimal medical therapy to reduce moderate or severe exacerbations throughout 1 year, compared with optimal medical therapy alone.


The study design is a multicenter, randomized, full sham bronchoscopy controlled, double-blind trial that will enroll 400 patients (1:1 randomization). Key inclusion criteria are FEV1/FVC < 0.7, FEV1 30 to 60% of predicted, post-bronchodilator, ≥ 2 moderate or 1 severe COPD exacerbations in the prior year, and COPD assessment test (CAT) ≥ 10. Primary objective will be the comparison of moderate or severe COPD exacerbations through 12 months of TLD therapy with optimal medical therapy versus optimal medical therapy alone. The sham group will be allowed to cross over at 1 year. Patients will be followed for up to 5 years.


The multicenter, randomized, full sham bronchoscopy controlled, double-blind AIRFLOW-3 trial will evaluate the efficacy of TLD to reduce moderate or severe COPD exacerbations beyond optimal medical therapy alone. The target population are patients with COPD, who suffer persistent symptoms and exacerbations despite optimal treatment, defining an unmet medical need requiring novel therapeutic solutions. This trial is registered at NCT03639051.

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COPD is characterized by persistent respiratory symptoms and airflow limitation due to airway and/or alveolar abnormalities [1]. COPD is a major cause of morbidity and mortality worldwide, and in the United States, costs associated with hospitalizations for exacerbations represent the largest proportion of total cost across all disease stages [2]. Reducing the risk for future exacerbations is a crucial guideline-directed goal of COPD management [1].

Inhaled pharmacologic treatments for COPD include drugs stimulating adrenergic receptors in airway smooth muscle (long-acting ß-agonists; LABA) or preventing acetylcholine binding to muscarinic receptors in the airways (long-acting muscarinic antagonists; LAMA) to induce bronchodilation, relax airway smooth muscle, and reduce airway inflammation [3]. LABA and LAMA also reduce exacerbation risk, and adding inhaled corticosteroids in dual or triple therapy may also enhance this effect in some patients [1, 4]. However, despite the benefits of inhaled pharmacologic treatments for COPD, a significant number of patients have persistent symptom and exacerbation burden (classified as GOLD Group “D”). Development of a therapeutic procedure that could reduce the risk for future exacerbation is an important research objective [5].

Baseline autonomic input of the vagus nerve, which modulates airway smooth muscle tone, mucus hypersecretion and hyperresponsiveness [6,7,8], is elevated in COPD [6]. Targeted lung denervation (TLD) aims to disrupt pulmonary nerve input to and from the lung to reduce clinical consequences of neural hyperactivity via improved bronchodilation, reduced mucous secretion of airway submucosal glands, and reduced airway hyperresponsiveness through disruption of pulmonary nerve reflexes [6,7,8]. Other potential impacts of TLD include disruption of other mediators of mucous secretion and inflammation such as neuropeptides [9]. Previous studies of TLD therapy have demonstrated proof of concept, evaluated dosing, established a safety profile, and identified potential efficacy outcomes [10,11,12]. Secondary analysis of AIRFLOW-2, a phase IIB safety multicenter study using a 1:1 randomized, sham-controlled, double-blinded design showed a statistically significant decrease in hospitalizations for COPD exacerbation with a trend toward significance for moderate-to-severe exacerbations [11]. Given these promising results, a prospective study of TLD therapy in a larger group of patients is warranted.

This paper describes the study design for A Multicenter, Randomized, Sham-controlled Study to Evaluate Safety and Efficacy After Treatment with the Nuvaira® Lung Denervation System in Subjects with Chronic Obstructive Pulmonary Disease (AIRFLOW-3). The primary objective of this study is to evaluate the efficacy of TLD to reduce moderate or severe (hospitalized) exacerbations of COPD beyond optimal medical therapy alone.



AIRFLOW-3 is a prospective, multicenter, randomized, sham-controlled, double-blind, safety and efficacy study designed to prospectively evaluate TLD’s impact on moderate-to-severe exacerbations in GOLD Group D patients. The AIRFLOW-3 subject profile is diagnosed COPD with FEV1 30–60% predicted, a documented history of at least 2 moderate or 1 severe exacerbation in the 12 months prior to consent, and persistent symptoms (CAT > 10) while on optimal medical treatment [1]. Up to 40 academic investigational centers are planned (approximately 25 US sites (> 60% subject participation)) and 15 sites in Europe (France, UK, Netherlands, Germany, Austria) and Canada (< 40% subject participation). Participants will be randomized (1:1) to TLD therapy plus optimal medical care (active treatment) or sham bronchoscopy procedure plus optimal medical care (sham control) utilizing a clinical electronic data capture (EDC) software. Randomization will be stratified based on site, participation in a pulmonary rehabilitation maintenance program, and baseline use of an inhaled corticosteroid at the time of enrollment. Stratification, which normalizes the impact of ICS and PR on patient outcomes, has no impact on the statistical power of the trial. The study is registered on (NCT03639051) and the protocol will have site ethics committee (EC) or Institutional Review Board (IRB) approval prior to any subject consent. The study will be conducted in accordance with Good Clinical Practice guidelines and all applicable country, state, and local regulations.

Primary outcome measures

AIRFLOW-3 is the first interventional COPD trial with a primary objective of reduction in moderate or severe (hospitalized) exacerbations compared with optimal medical care alone. For the purpose of enrollment and follow-up in this study, a COPD exacerbation will be defined as a complex of respiratory events/symptoms (increase or new onset) of more than one of the following: cough, sputum, wheezing, dyspnea or chest tightness with at least one symptom with a duration of at least 3 days and requiring treatment with antibiotics and/or corticosteroids (moderate exacerbation) and including hospital admission or emergency room / acute care visit > 24 h in duration (severe exacerbation) [13]. COPD exacerbations will be determined by and treated at the discretion of the Investigator in accordance with guideline-based recommendations.

To assess the primary objective, the primary endpoint is a comparison of time-to-first event for moderate or severe COPD exacerbations between the active treatment arm and the sham control arm based on a log-rank test. Event timing will be based on the time from the date of randomization to the date of a patient’s first primary endpoint event, or to the close of the 12-month visit window for patients who do not experience a primary endpoint event. Patients who have not experienced a primary endpoint event and are lost to follow-up, or withdrawn, prior to the close of the 12-month visit window, will be censored at the date of their last known status.

Secondary outcome measures

Secondary outcome measures will include comparisons between study arms of time to first respiratory-related hospitalization and time to first severe exacerbation at 12 months. Other prespecified secondary outcome measures include the difference between study and control group at 12 months for: quality of life (St. George’s Respiratory Questionnaire COPD version (SGRQ-C) scores, CAT scores, short form health survey (SF-36) scores), transitional dyspnea index (TDI), and changes in spirometric (FEV1 and FVC) and plethysmographic (RV) lung volume measures, see Table 1.

Table 1 Primary and Secondary Endpoints


A sub-study on airway inflammatory biomarkers will be offered to randomized subjects at participating AIRFLOW-3 centers. Bronchial brushes will be collected at the time of the study procedure and during a second airway inspection at 6-months post-procedure. Three brushes from the right lower lobe segmental bronchi will be collected and analyzed. The study is exploratory in nature and gene expression changes after TLD will be based on transcriptome analysis; including differentially expressed genes, cluster analysis, and gene set enrichment [14].

Patient recall and recruitment

Monthly phone-visit follow-ups are planned for months when an in-person follow-up visits do not occur. A memory aid to record daily symptoms of exacerbation and medications will be provided to support patient recall of changes in respiratory symptoms, medications, and any respiratory-related healthcare resource utilization for the first 12 months.

Study sites may advertise for local recruitment. Study information and/or slides for presentation can be provided to referring physicians upon request. IRB/EC approval of any materials to be used for direct patient recruitment will be obtained by the reviewing IRB/EC prior to use.

Screening assessments

A summary of participant flow from consent to study exit is detailed in Fig. 1 and required testing and assessments are included in Additional file 1. Participants will be considered enrolled at the time of consent. Following consent, participants will undergo baseline screening to assess eligibility including medical history. Inclusion/exclusion criteria for the study are outlined in detail in Tables 2 and 3. Participants must have documented exacerbation history of at least 2 moderate COPD exacerbations or 1 severe COPD exacerbation in the 12-months prior to enrollment while on optimal maintenance COPD medications (minimum 12 months on LABA/LAMA therapy, or similar pharmacologic regimen). All participants will have an inspiratory chest CT scan submitted to the core lab for exclusionary review and confirmation of appropriate airway sizing prior to treatment (Fig. 2).

Fig. 1

Participant flow through the study

Table 2 Inclusion Criteria
Table 3 Exclusion Criteria
Fig. 2

Example of transverse and coronal slice CT airway measurements. The patient’s computed tomography scan of the chest is reviewed prior to the procedure to re-confirm proper airway sizes and geometry. The right mainstem bronchial length must be ≥10 mm to ensure an adequate location for electrode placement. The diameters measured on the transverse scan and coronal scan (diameter indicated by white dotted lines on CT images) must be averaged to determine the appropriate catheter size for both mainstem bronchi

A cardiac assessment, including an ECG and medical clearance for anesthesia, will be required as part of baseline screening. To exclude patients with gastrointestinal symptoms, the validated, patient-reported gastroparesis cardinal symptom index (GCSI) assessment [15] will be administered. Scores of ≥18.0 on this index will be exclusionary.

Respiratory medication

Since the primary objective of this study compares TLD plus optimal medical care to optimal medical care alone, it will be important to document and control respiratory medications from the time of consent through the primary endpoint analysis period unless there is a drug specific adverse event requiring discontinuation. For the purposes of this study, optimal medical care is recommended per the GOLD 2019 guidelines [1]. Participants already taking an inhaled corticosteroid (ICS) or other classes of medication at the time of consent should continue taking them through the one-year study exit visit, to avoid potential confounding impact of medication changes. Randomization will be stratified to ensure an equal distribution of ICS patients in the sham and treatment arms. When necessary, changes in COPD medications are permitted for a legitimate medical need to protect the subject and will not be documented as a protocol deviation. All medication changes will be closely monitored and recorded for the duration of the study.

Blinding and group allocations

The study blinding plan will be implemented at each of the sites to ensure that double-blinding is maintained throughout the 12-month follow-up period. Blinded (follow-up visits) and unblinded (study procedure) teams will be formed at each center. All sham and TLD procedures will be performed by a physician. Participants randomized to the sham group will undergo a sham TLD procedure with the Nuvaira lung denervation system (the esophageal balloon and dNerva® catheter will be placed and the balloon inflated but no fluoroscopy or radiofrequency (RF) energy will be delivered). Steps will be taken to ensure that the bronchoscopy suite is staged, and equipment manipulated in a manner that provides a similar total procedure experience regardless of treatment allocation. Of note, TLD yields no radiographically visible implants or treatment evidence. Following the 12-month follow-up period of double-blinding, participants in the sham group will be offered the opportunity to undergo TLD therapy, followed for up to 4 years and evaluated as a cross over group.

The treatment group will undergo active TLD treatment with the Nuvaira lung denervation system (fluoroscopy and RF energy will be delivered). TLD is delivered via a dual-cooled balloon catheter as previously described, see Fig. 3 [10,11,12, 16]. The Nuvaira catheter is passed through the working channel of a 3.2 mm flexible bronchoscope and coupled with the bronchoscope. This provides direct visualization of the catheter, balloon, and electrode-tissue interface and guides proper axial positioning along the length of the bronchi. Fluoroscopy is used to guide the proper rotational positioning and the electrode distance from the esophagus. Complete circumferential treatment involves 4 quadrant rotations of the catheter in each main bronchus. Both lungs are treated in a single procedure, with an average of one or two catheters used, depending on airway dimension. The procedure steps are described in Fig. 4.

Fig. 3

Image of the Nuvaira Console with the dNerva Catheter in bronchoscope and the expandable cooled balloon. a An image of the Nuvaira lung denervation system including the Nuvaira Console and dNerva Catheter. b The dNerva catheter is inserted through the working channel of a flexible bronchoscopes and inflated following positioning. Cooling fluid is circulated through the catheter by the console and provides the cooling that protects the airway wall during energy delivery (blue arrows indicate fluid flow). c During the procedure, the catheter is positioned in the mainstem bronchi and d visualization of the electrode positioning is confirmed by coupling the bronchoscope to the distal end of the catheter balloon

Fig. 4

Key steps of the targeted lung denervation procedure. All four steps are repeated until the entire circumference of the first bronchus is treated to ablate the nerves. This is typically achieved in 4 activations. The catheter is then retracted, and the opposite main bronchus treated

At each site, the first 3 enrolled participants will be treated with TLD therapy (no randomization) as “roll-in cases” designed to evaluate the procedural learning curve. These patients will undergo the same pre- and post-treatment assessments as randomized subjects but will be analyzed separately from the randomized cohort. All subjects will be followed for 5 years.

Statistical analysis

Primary endpoint analyses will be conducted on the intent-to-treat (ITT), randomized population.

Secondary endpoints (including adverse events) will be based on a modified ITT population (all participants for whom the active procedure or the sham procedure is initiated, excluding roll-in procedure participants). One-sided statistical tests will be considered significant at p-values less than 0.025 while two-sided tests will be significant at p-values less than 0.05. The statistical test for the primary endpoint will be based on a log-rank test, comparing the survival distribution of the time-to-first event for the primary endpoint. The secondary endpoints will be tested using a combination of sequential gatekeeping procedure and Hommel adjustment to control for type I error rate.

Sample size for the study was driven by the number of primary endpoint events required to obtain adequate power and based on event-rate data from AIRFLOW-2. Assuming the percentage of participants with primary endpoint events through 12 months is 65 and 48.75% for the sham control and TLD groups respectively, a sample size of 400 will provide greater than 90% power based on a two-sided 0.05 alpha level log-rank test. An attrition rate of 10% at 12-months has been accounted for in the sample size calculation.


Oversight of the overall conduct of the study is the responsibility of the Steering Committee, which includes, but is not limited to, dissemination of data (including publications), recommendations of the independent data monitoring committee (DMC), and ongoing review of safety data. The DMC will provide independent monitoring of the study. The DMC will be charged with monitoring the overall study for safety, invoking study stopping rules, and for auditing the quality of the data. Treatment safety will be assessed by monitoring the incidence of all adverse events (AEs), serious adverse events (SAEs), and all unanticipated adverse device effects (UADEs) from randomization through 12 months. An independent Clinical Events Committee will adjudicate all AEs [1].

Long-term safety will be assessed by monitoring the incidence of a prospectively defined subset of important respiratory, cardiovascular, and gastrointestinal SAEs, and all-cause mortality out to 5 years. Sham control participants who are treated after the first year of follow-up will be included in the assessment of long-term safety.

Study timeline

The study will begin in May of 2019 and final follow-up of the primary endpoint is expected to be completed in August of 2022.

Study organization

This study was designed and guided by the study steering committee which consisted of 2 principal investigators and 5 physicians, working in academic hospital settings. Electronic data are collected at the study sites; data transfer, management and storage, quality control rest on Nuvaira, Inc. The AIRFLOW-3 trial is fully sponsored by Nuvaira, Inc. USA.


Continued exacerbation events in guideline treated patients remains a therapeutic challenge in COPD management [17,18,19]. Acute COPD exacerbations are associated with a rapid decline in lung function and with impaired survival [20], mortality in the year following a severe hospitalized exacerbation is estimated to be as high as 21% [21]. In the US, exacerbation-related costs represent the highest proportion of total COPD costs, across all levels of disease severity [22].

This study requires that patients be taking a minimum of two bronchodilators (i.e. stable GOLD guideline drug therapy) for 12 months prior to randomization and strongly recommends no maintenance therapy changes during the 12-month period following randomization. Maintenance add-on therapies (i.e., ICS, PDE4 inhibitors and azithromycin) are allowed at the discretion of the treating physician. All patient drug use will be tracked and recorded throughout the randomization period. Additionally, patients using an ICS will be stratified evenly between the treatment and sham groups [23].

Pulmonary rehabilitation (PR) improves dyspnea, health status, and exercise tolerance in stable patients, although PR appears to have no measurable impact on the risk of COPD exacerbation [24]. However, PR is under-utilized in COPD, particularly in the US [25]. Therefore, a requirement for PR program completion, while ideal, would result in a potential impediment to enrollment for significant numbers of otherwise qualified subjects. AIRFLOW-3 will thus record patient experience with PR (naïve or past participation) at baseline and throughout the trial, but will not require pulmonary rehabilitation as an inclusion criterion. Rather, subjects will be stratified based on their prior PR experience.

Time-to-first event analysis is considered the most robust way to measure COPD exacerbations in clinical studies because it is unlikely to be affected by early patient exits and associated missing data [26]. Analysis of the proportion of patients experiencing at least one exacerbation event is also important particularly in the context of individual risk-reward preferences. Recently published, large-scale randomized controlled trials targeting reduction in COPD exacerbation have documented that up to 65% of patients experience at least one moderate or severe exacerbation over 12 months of study follow-up [19, 23]. Percent reduction in total number of exacerbations can generate a large between-group difference skewed by a small number of patients with a disproportionately high number of events [27]. Therefore, the primary endpoint for AIRFLOW-3 is the time-to-first event analysis of the proportion of patients experiencing one or more moderate or severe exacerbations, comparing the active treatment (TLD) to the sham-control arm.

Increasingly, the COPD literature examining the effects of pharmacological treatment suggests that there are only modest alterations in secondary outcomes associated with clinically meaningful reductions in exacerbations [23, 28]. The SPARK trial comparing dual with single maintenance bronchodilator therapy (tiotropium) reported a 12% reduction in all moderate or severe exacerbation events favoring dual therapy, yet clinically unimportant changes in FEV1 (increased 60 vs. 80 mL) and SGRQ-C (decreased − 1.7 vs. -3.1) between groups [28]. In IMPACT, the 6.8% relative reduction in the proportion of patients with a moderate or severe COPD exacerbation was associated with differences of 54 mL in FEV1 and a − 1.8 in SGRQ-C between groups [23]. Such discrepancies between changes in exacerbation events and changes in baseline symptoms or lung function is biologically and medically consistent with a potential mechanism of TLD, that being disruption of reflex airway reactivity. AIRFLOW-3 will explore the impact of treatment on secondary outcomes such as FEV1 and SGRQ-C.

In conclusion, the AIRFLOW-3 trial will evaluate the efficacy of TLD to reduce moderate or severe COPD exacerbations beyond optimal medical treatment. Earlier-phase trials have demonstrated feasibility and a positive safety profile of TLD in COPD patients out to 3-years post-treatment. The target population is GOLD Group D patients, who suffer persistent symptoms and exacerbations despite optimal guideline-directed therapy, defining an unmet medical need requiring a novel therapeutic solution.

Availability of data and materials

Not applicable.



Adverse event


COPD assessment test


Chronic obstructive pulmonary disease


Data monitoring committee


Ethics committee


Gastroparesis cardinal symptom index


Global Initiative for Chronic Obstructive Lung Disease


Inhaled corticosteroid


Institutional review board




Long-acting ß-agonist


Long-acting muscarinic antagonist


Pulmonary function testing


Pulmonary rehabilitation


Targeted lung denervation


  1. 1.

    Global Initiative for Chronic Obstructive Lung Disease (GOLD). 2019 Global strategy for prevention, diagnosis and management of COPD [].

  2. 2.

    Chronic obstructive pulmonary disease (COPD) [].

  3. 3.

    Riley CM, Sciurba FC. Diagnosis and outpatient Management of Chronic Obstructive Pulmonary Disease: a review. JAMA. 2019;321(8):786–97.

    Article  Google Scholar 

  4. 4.

    Tashkin DP, Celli B, Senn S, Burkhart D, Kesten S, Menjoge S, Decramer M, Investigators US. A 4-year trial of tiotropium in chronic obstructive pulmonary disease. N Engl J Med. 2008;359(15):1543–54.

    CAS  Article  Google Scholar 

  5. 5.

    Rothnie KJ, Mullerova H, Smeeth L, Quint JK. Natural history of chronic obstructive pulmonary disease exacerbations in a general practice-based population with chronic obstructive pulmonary disease. Am J Respir Crit Care Med. 2018;198(4):464–71.

    Article  Google Scholar 

  6. 6.

    Canning BJ. Reflex regulation of airway smooth muscle tone. J Appl Physiol (1985). 2006;101(3):971–85.

    CAS  Article  Google Scholar 

  7. 7.

    Zaccone EJ, Undem BJ. Airway vagal neuroplasticity associated with respiratory viral infections. Lung. 2016;194(1):25–9.

    CAS  Article  Google Scholar 

  8. 8.

    Rogers DF. Motor control of airway goblet cells and glands. Respir Physiol. 2001;125(1–2):129–44.

    CAS  Article  Google Scholar 

  9. 9.

    Atanasova KR, Reznikov LR. Neuropeptides in asthma, chronic obstructive pulmonary disease and cystic fibrosis. Respir Res. 2018;19(1):149.

    Article  Google Scholar 

  10. 10.

    Slebos DJ, Klooster K, Koegelenberg CF, Theron J, Styen D, Valipour A, Mayse M, Bolliger CT. Targeted lung denervation for moderate to severe COPD: a pilot study. Thorax. 2015;70(5):411–9.

    Article  Google Scholar 

  11. 11.

    Slebos DJ, Shah PL, Herth FJ, Pison C, Schumann C, Hubner RH, Bonta PI, Kessler R, Gesierich W, Darwiche K, et al. Safety and adverse events after targeted lung denervation for symptomatic moderate to severe COPD (AIRFLOW): a multicenter randomized controlled trial. Am J Respir Crit Care Med. 2019;200:1477.

    Article  Google Scholar 

  12. 12.

    Valipour A, Asadi S, Pison C, Jondot M, Kessler R, Benneddif K, Deslee G, Verdier M, Slebos DJ, Mayse M. Long-term safety of bilateral targeted lung denervation in patients with COPD. Int J Chron Obstruct Pulmon Dis. 2018;13:2163–72.

    CAS  Article  Google Scholar 

  13. 13.

    Vogelmeier C, Hederer B, Glaab T, Schmidt H, Rutten-van Molken MP, Beeh KM, Rabe KF, Fabbri LM, Investigators P-C. Tiotropium versus salmeterol for the prevention of exacerbations of COPD. N Engl J Med. 2011;364(12):1093–103.

    CAS  Article  Google Scholar 

  14. 14.

    Kistemaker LE, Slebos DJ, Meurs H, Kerstjens HA, Gosens R. Anti-inflammatory effects of targeted lung denervation in patients with COPD. Eur Respir J. 2015;46(5):1489–92.

    Article  Google Scholar 

  15. 15.

    Revicki DA, Rentz AM, Dubois D, Kahrilas P, Stanghellini V, Talley NJ, Tack J. Gastroparesis cardinal symptom index (GCSI): development and validation of a patient reported assessment of severity of gastroparesis symptoms. Qual Life Res. 2004;13(4):833–44.

    Article  Google Scholar 

  16. 16.

    Valipour A, Shah PL, Pison C, Ninane V, Janssens W, Perez T, Kessler R, Deslee G, Garner J, Abele C, et al. Safety and dose study of targeted lung denervation in moderate/severe COPD patients. Respiration. 2019;98:1–11.

    Article  Google Scholar 

  17. 17.

    Halpin DMMM, Metzdorf N, Celli B. Impact and prevention of severe exacerbations of COPD: a review of the evidence. Int J Chron Obstruct Pulmon Dis. 2017;12:2891–908.

    Article  Google Scholar 

  18. 18.

    Criner GJ, Connett JE, Voelker H. Simvastatin in moderate-to-severe COPD. N Engl J Med. 2014;371(10):970–1.

    PubMed  Google Scholar 

  19. 19.

    Pavord ID, Chanez P, Criner GJ, Kerstjens HAM, Korn S, Lugogo N, Martinot JB, Sagara H, Albers FC, Bradford ES, et al. Mepolizumab for Eosinophilic chronic obstructive pulmonary disease. N Engl J Med. 2017;377(17):1613–29.

    CAS  Article  Google Scholar 

  20. 20.

    Han MK, Quibrera PM, Carretta EE, Barr RG, Bleecker ER, Bowler RP, Cooper CB, Comellas A, Couper DJ, Curtis JL, et al. Frequency of exacerbations in patients with chronic obstructive pulmonary disease: an analysis of the SPIROMICS cohort. Lancet Respir Med. 2017;5(8):619–26.

    Article  Google Scholar 

  21. 21.

    McGhan R, Radcliff T, Fish R, Sutherland ER, Welsh C, Make B. Predictors of rehospitalization and death after a severe exacerbation of COPD. Chest. 2007;132(6):1748–55.

    Article  Google Scholar 

  22. 22.

    Guarascio AJ, Ray SM, Finch CK, Self TH. The clinical and economic burden of chronic obstructive pulmonary disease in the USA. Clinicoecon Outcomes Res. 2013;5:235–45.

    PubMed  PubMed Central  Google Scholar 

  23. 23.

    Lipson DA, Barnhart F, Brealey N, Brooks J, Criner GJ, Day NC, Dransfield MT, Halpin DMG, Han MK, Jones CE, et al. Once-daily single-inhaler triple versus dual therapy in patients with COPD. N Engl J Med. 2018;378(18):1671–80.

    CAS  Article  Google Scholar 

  24. 24.

    Puhan MA, Gimeno-Santos E, Cates CJ, Troosters T. Pulmonary rehabilitation following exacerbations of chronic obstructive pulmonary disease. Cochrane Database Syst Rev. 2016;12:CD005305.

    PubMed  Google Scholar 

  25. 25.

    Spitzer KA, Stefan MS, Priya A, Pack QR, Pekow PS, Lagu T, Pinto-Plata VM, ZuWallack RL, Lindenauer PK. Participation in pulmonary rehabilitation after hospitalization for chronic obstructive pulmonary disease among Medicare beneficiaries. Ann Am Thorac Soc. 2019;16(1):99–106.

    Article  Google Scholar 

  26. 26.

    Wedzicha JA, Decramer M, Seemungal TA. The role of bronchodilator treatment in the prevention of exacerbations of COPD. Eur Respir J. 2012;40(6):1545–54.

    Article  Google Scholar 

  27. 27.

    Beeh KM, Hederer B, Glaab T, Muller A, Rutten-van Moelken M, Kesten S, Vogelmeier C. Study design considerations in a large COPD trial comparing effects of tiotropium with salmeterol on exacerbations. Int J Chron Obstruct Pulmon Dis. 2009;4:119–25.

    CAS  PubMed  PubMed Central  Google Scholar 

  28. 28.

    Wedzicha JA, Decramer M, Ficker JH, Niewoehner DE, Sandstrom T, Taylor AF, D'Andrea P, Arrasate C, Chen H, Banerji D. Analysis of chronic obstructive pulmonary disease exacerbations with the dual bronchodilator QVA149 compared with glycopyrronium and tiotropium (SPARK): a randomised, double-blind, parallel-group study. Lancet Respir Med. 2013;1(3):199–209.

    CAS  Article  Google Scholar 

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This study is sponsored by Nuvaira, Inc., which is also responsible for data collection. Statistical services will be provided by Nuvaira, Inc. and NAMSA, VIDA Diagnostics for CT measurements, and Aquilo for sub-study analysis. The authors thank Heather Gorby, PhD for providing medical writing support and Nuvaira, Inc. for contribution to the study design and management of the study, as well as the development and management of the study data base. The authors decided to submit this manuscript for publication.

Trial sponsor

Nuvaira, Inc. 3750 Annapolis Lane North, Suite 105. Minneapolis, MN, 55447 USA.

Phone: + 1.763.450.2800.



No external funding, i.e., grants or governmental funds, were awarded for support of this study.

Author information





DS, BD, AV, PS, GD and FS contributed equally to study concept and design, drafting and critical revision of the manuscript for important intellectual content. DS, BD, AV, PS, GD and FS read and approved the final manuscript.

Corresponding author

Correspondence to Dirk-Jan Slebos.

Ethics declarations

Ethics approval and consent to participate

The study (Protocol D0543, dated 31 May 2019) is designed in accordance with the Declaration of Helsinki and approved by the Ethics Committee of the City of Vienna (Austria), Comité de Protection des Personnes (CPP) OUEST II – Angers (France), Ethics Committee of the Faculty of Heidelberg (Germany), University Medical Center Groningen (UMCG) Medical Ethics Review Board (Netherlands), East of England – Cambridge East Research Ethics Committee (United Kingdom), Western Institutional Review Board (WIRB; United States) and John Hopkins Medical Institutional Review Board (United States). Written informed consent will be obtained from all participants.

Consent for publication

The ethical approval and patient information include consent to publish collected data.

Competing interests

BD reports grants and personal fees from Novartis, Actelion and consultation fees from Nuvaira, PneumRx, Teva, GSK, AstraZeneca, Chiesi, Menarini and Boehringer Ingelheim France. GD has received consultation fees from Nuvaira. All clinical trial activities are sponsored by Nuvaira, Inc.

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Supplementary information

Additional file 1.

Required Testing and Assessments.

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Slebos, D., Degano, B., Valipour, A. et al. Design for a multicenter, randomized, sham-controlled study to evaluate safety and efficacy after treatment with the Nuvaira® lung denervation system in subjects with chronic obstructive pulmonary disease (AIRFLOW-3). BMC Pulm Med 20, 41 (2020).

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  • COPD
  • Nerves
  • Targeted lung denervation
  • Acetylcholine
  • Anticholinergic
  • Bronchoscopy