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  • Research article
  • Open Access
  • Open Peer Review

Implementation of a successful eradication protocol for Burkholderia Cepacia complex in cystic fibrosis patients

  • 1,
  • 1,
  • 1,
  • 1,
  • 1, 2,
  • 1, 2,
  • 1, 2,
  • 1, 2 and
  • 1, 2Email author
BMC Pulmonary MedicineBMC series – open, inclusive and trusted201818:35

https://doi.org/10.1186/s12890-018-0594-8

  • Received: 3 December 2017
  • Accepted: 24 January 2018
  • Published:
Open Peer Review reports

Abstract

Background

Infection with Burkholderia cepacia complex (Bcc) results in a heterogeneous clinical course ranging from asymptomatic colonization of the airways to fulminant respiratory failure in patients with cystic fibrosis (CF). Early eradication of Pseudomonas aeruginosa improves clinical outcomes. The efficacy and clinical outcomes following implementation of an eradication protocol for Bcc are less well understood.

Methods

We developed and implemented a single center Bcc eradication protocol that included an intensive combination of intravenous, inhaled, and oral antibiotic therapies based on in vitro sensitivities. We conducted a retrospective cohort analysis of clinical outcomes compared to patients with chronic Bcc infection.

Results

Six patients were identified as having a newly acquired Bcc colonization and were placed on the eradication protocol. Sequential sputum samples after completion of the protocol demonstrated sustained clearance of Bcc in all patients. Lung function and nutritional status remained stable in the year following eradication.

Conclusion

Clearance of Bcc from sputum cultures using a standardized protocol was successful at one year and was associated with clinical stability.

Keywords

  • Burkholderia cepacia
  • Burkholderia Cepacia complex (BCC)
  • Cystic fibrosis
  • Infection eradication

Background

Cystic Fibrosis (CF) is characterized by development of viscous airway mucus which provides a fertile media for opportunistic respiratory pathogens [1]. Chronic respiratory infection with recurrent exacerbations and inflammation results in progressive bronchiectasis and ultimately respiratory failure [2, 3]. Aggressive management of chronic respiratory infections is crucial to prevent lung function decline. Pseudomonas aeruginosa is a common respiratory pathogen in the CF airway and chronic colonization is associated with impaired lung function for these patients [4, 5]. Early eradication upon initial positive sputum culture with P. aeruginosa has been shown to stabilize lung function and improve clinical outcomes with successful eradication ranging from 62 to 93% although the long-term clinical benefit may be uncertain [68].

Though less prevalent than P. aeruginosa, CF patients may acquire other respiratory pathogens including species of the Burkholderia cepacia complex (Bcc) [9]. Bcc represents a group of genetically related bacteria associated with a heterogeneous clinical course ranging from asymptomatic colonization to fulminant respiratory failure [10, 11]. Chronic colonization with Bcc is associated with antibiotic resistance, increased risk of respiratory failure, and worsened mortality [10, 12]. Although Bcc eradication has been previously described, implementation of a protocolized approach toward Bcc eradication has not been fully studied [13, 14].

Methods

Patient identification and data collection-

We obtained institutional review board approval to conduct a retrospective chart review of CF patients infected with Bcc at our center. Beginning January 2012 through June 2015, patients were placed on the eradication protocol if they were found to have a sputum culture positive for Bcc, which had not been isolated from sputum during the two prior years. Patients who were known to have chronic airway colonization by Bcc were treated with guideline-based standard of care [15]. Lung function (forced expiratory volume in one second, FEV1% predicted), nutritional status (Body mass index, BMI), and serial sputum cultures were obtained during clinic appointments that generally occurred at 3-month intervals, per CF guidelines. The genomovar of Bcc strains isolated was confirmed at the B. cepacia Research Laboratory and Repository at the University of Michigan. Successful eradication of Bcc was defined as clearance of Bcc following the induction stage of therapy with maintenance of at least three additional negative respiratory cultures at 3-month intervals during the 12-month follow-up.

Treatment protocol

Patients included in the eradication group following sputum culture isolation of newly acquired Bcc were placed on the eradication protocol (Table 1). Antibiotic selection was based on identifying an aggressive combination of inhaled and intravenous therapies with broad coverage to obtain maximal in-vivo treatment synergy as multiple patient strains demonstrated pan-resistance based on in-vitro antibiotic sensitivity testing. Tobramycin was included for empiric coverage of P. aeruginosa given the high rate of co-infection in this population. The rationale for antibiotic selection is included in Table 1.
Table 1

Burkholderia Eradication Protocol

 

Medication

Dosing

Frequency

Route

Bacteria Targeted

Induction Period (21 Days):

Tobramycin

6 mg/kg (per kinetics)

Daily

IV

PsA, Bcc

Ceftazidime

2 g

Every 8 h

IV

PsA, Bcc

Trimethoprim/Sulfamethoxazole

800/160 mg

Twice Daily

Oral

Bcc

Tobramycin inhaleda

300 mg

Twice Daily

Nebulized

PsA, Bcc

Azithromycin

250 mg

Daily

Oral

Anti-inflammatory

Consolidation Period (2 months):

Trimethoprim/Sulfamethoxazole

800/160 mg

Twice daily

Oral

Bcc

Tobramycin inhaleda

300 mg

Twice daily

Nebulized

PsA, Bcc

Azithromycin

250 mg

Daily

Oral

Anti-inflammatory

aAlternative- TIP (tobramycin dry powder for inhalation, 4 caps (28 mg/cap)) every 12 h

Statistical analysis

Statistical analysis was performed using GraphPad Prism version 6.0c (GraphPad Software, Inc., San Diego, CA). Continuous variables were described using means with standard deviations, or medians with ranges as sample sizes were small, and were analyzed using Mann-Whitney t-test or Two-way ANOVA testing as data were non-parametric. Change in lung function and nutritional status was compared between groups using Two-way ANOVA; Fisher’s post hoc test was used when ANOVA was significant. All statistical tests were two-sided and P-values < 0.05 were considered statistically significant.

Results

Patient cohort

From January 2012 to June 2015, 14 adult patients were found to have Bcc isolated in sputum cultures. Seven of these patients were found to have a newly acquired Bcc colonization (no isolation of Bcc from sputum in the two preceding years), of which six were placed on eradication protocol. The remaining patient was not included in analysis due to a new acquisition of P. aeruginosa during the maintenance phase of the Bcc eradication, requiring transition to a PsA eradication protocol. Seven additional patients were defined as having chronic colonization with Bcc based on prior positive cultures for Bcc; these patients were treated based on guidelines for pulmonary exacerbation and were considered as contemporaneous controls. Of these seven control patients, two patients were not included due to inadequate clinic follow up. A third patient with Bcc colonization was not included due to identification of only one CF causing mutation despite genetic sequencing. These patients were excluded from further analysis. This control group thus consists of patients with chronic Bcc infection and act as case controls for patients with acute infection.

Outcome following bcc Eradication

Demographic data for the two groups including age, gender, CFTR genotype, baseline lung function, nutritional status, pancreatic functional status, and microbiology culture results at baseline were collected via chart review (Table 2). All patients in both groups were maintained on thrice weekly oral azithromycin therapy for anti-inflammatory effect. In addition, all patients placed on Bcc eradication were previously colonized with PsA and were maintained on every-other-month inhaled tobramycin. Three of the four control patients were also previously maintained on inhaled tobramycin for chronic PsA infection.
Table 2

Baseline characteristics

Group

Gender

Age

Genotype (n)

Pancreatic Insufficient (%)

CFRD (%)

Bcc Species Isolated (n)

Additional Pathogens (n)

FEV1% Predicted

BMI

Eradication

(n = 6)

50% F

23.3 (20–27)

dF508/dF508 [6]

(100%)

83%

B. multivorans [4]

B. gladioli [2]

B. cepacia [1]

PsA [6]

MRSA [3]

Steno [2]

Aspergillus [2]

65.5 (30–96%)

20.2 (15.6–26.9)

Usual Care

(n = 4)

50% F

28.2 (21–47)

dF508/dF508 [1]

405 3AtoC/V317A Q98X [1]

dF508/Q98X [1]

dF508/G542X [1]

(75%)

75%

B. multivorans [1]

B. cepacia [2]

B. arboris [1]

PsA [3]

MRSA [4]

55.7 (31–98%)

20.3 (18.9–22)

Abbreviations: F=Female, CFRD- CF Related Diabetes, MRSA- Methicillin Resistant Staphylococcus aureus, PsA- Pseudomonas aeruginosa, Steno- Stenotrophomonas maltophilia

At baseline there was no significant difference between the group means of the eradication and control groups for FEV1% predicted (65.5 ± 24.5% vs. 59.7 ± 28.2%, respectively, p = 0.67) or BMI (20.2 ± 3.8 vs. 20.3 ± 1.2, respectively, p = 0.94). Among patients in the eradication group, 100% of the patients remained free of Bcc at the end of the study compared to 25% in the control group. During the one-year follow up period the eradication group experienced a mean number of exacerbation of 1.66 ± 0.42 versus 3.25 ± 1.0 by the control group (p = 0.166). The mean time to first exacerbation was 230 days (± 24.55) in the eradication group versus 88 ± 27.6 in the control group (p = 0.009). The change from baseline between the eradication and control groups was compared and at three months the groups had a significant difference in change of FEV1% predicted (10.8 ± 10.7% vs. -6.0 ± 8.8%, p = 0.01) (Fig. 1). No significant difference in change of FEV1% predicted was identified between the eradication and control groups at 6 (1.3 ± 13.0% vs. -7.5 ± 7.5%) or 12 months (− 2.6 ± 15.1% vs. -10.2 ± 11.1%). With regards to nutritional status, no significant difference was identified in change in BMI from baseline between the eradication and control groups at three (0.68 ± 0.71 vs. 0.3 ± 0.64 kg/m2), six (0.56 ± 1.1 vs. -0.95 ± 1.6 kg/m2), or 12 months (1.8 ± 2.0 vs. 1.3 ± 0.5 kg/m2).
Fig. 1
Fig. 1

a. Change in mean lung function (FEV1% predicted) from baseline over time during the course of the cohort study for patients in the eradication group compared to patients treated with standard of care. *p = 0.01. b. Change in mean BMI from baseline for the same period

We also report absolute FEV1% and BMI in the eradication group by individual. Among these patients, no significant difference was seen in mean (± standard deviation) FEV1% predicted at three (76.5 ± 15.0), six (67 ± 20.9), and 12 months (63 ± 24.1) following eradication compared to either baseline (65.6 ± 24.4) or the average of the year prior to Bcc acquisition (74.3 ± 15.6). Similarly, no significant difference was seen in BMI at three (20.9 ± 4.1), six (20.8 ± 3.6), and 12 months (22.1 ± 4.4) compared to baseline (20.2 ± 3.8) or the average of the year prior to Bcc acquisition (20.4 ± 3.1) (Fig. 2).
Fig. 2
Fig. 2

a. Trends in lung function (FEV1% predicted) for each eradication group subject over time (n = 6 patients). b. Trends in nutritional status (BMI) for each eradication group subject over time (n = 6 patients). Dashed lines represent the trend of the group mean over time in each graph

Discussion

We describe the implementation of a Bcc eradication protocol for CF patients with a newly acquired Bcc colonization using an aggressive treatment regimen with a high success rate, suggesting its potential for benefit in this small group. Although no significant difference in lung function or BMI was observed at one year of follow-up between the eradication and control groups, the eradication group demonstrated universal Bcc clearance (100% as compared to 25% in the control group), stable lung function and nutritional status, and no treatment-related adverse events. We recognize the limitations of the small chronic Bcc case controls. This data is illustrative that chronically infected patients may have greater risk for exacerbation as well as increased CF-related morbidity. However, we acknowledge that these comparisons are not directly associated with the interventions outlined in our protocol. The protocol was relatively unbiased to patient selection, as all patients with newly discovered Bcc entered the protocol, with the exception of a single patient who was actively being treated for additional pulmonary pathogens, which precluded utilizing certain antibiotics.

It is important to note that a subset of patients, particularly younger patients, have been shown to spontaneously clear Bcc from sputum cultures suggesting that aggressive eradication may not always be necessary [13, 16]. Although it is not clear which patients are most likely to benefit from Bcc eradication, early eradication is likely to be clinically significant given the general association between Bcc colonization and progressive clinical decline [10, 17].

The protocol was, by intention, aggressive and it is not known whether this intensive therapy is required for successful Bcc eradication. Also note, this protocol demonstrated successful eradication of three different Bcc strains, some of which have not been previously associated with outcomes similar to an epidemic strain. Alternative protocols in pediatric patients with newly isolated Bcc have been described with some success and have utilized similarly aggressive regimens combining inhaled and intravenous Tobramycin, Ceftazidime and Temocillin [18]. The patients described by that series were not complicated by chronic PsA co-infection. An additional case-series described combination of inhaled amiloride and tobramycin with eradication of initial colonization in 3 of 4 patients but was unsuccessful in chronically infected patients [19, 20].

Conclusion

This study has several limitations including a small sample size, retrospective single-center design, and a control group that consisted of chronically infected patients who might have a different course of disease progression. Therefore, a multi-center, prospective study is warranted to evaluate the efficacy of aggressive Bcc eradication and provide a more definitive evaluation for treatment of this severe complication of CF lung disease and associated potential benefits on CF-related morbidity and exacerbation frequency we observed compared to case controls.

Abbreviations

Bcc

Burkholderia cepacia complex

BMI: 

Body mass index

CF: 

Cystic fibrosis

FEV1

Forced expiratory volume in 1 s

PsA: 

Pseudomonas aeruginosa

Declarations

Acknowledgments

None applicable.

Funding

No funding source was utilized for the current study.

Availability of data and materials

The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.

Summary at a glance

Burkholderia cepacia complex (Bcc) results in worsened clinical course in cystic fibrosis patients. Previously described Bcc eradication protocols have resulted in limited success. We describe an intensive Bcc eradication protocol that resulted in a high rate of Bcc-free cultures for one year of follow up.

Authors’ contributions

BG participated in study design, data collection and analysis, initial manuscript production, editing, and final manuscript. JC, DG, TS participated in data collection, manuscript editing, and final manuscript production. AG, KL, VA, SMR, and GMS participated in data analysis, initial manuscript preparation, manuscript editing, and final manuscript production. All authors read and approved the final manuscript.

Ethics approval and consent to participate

Approval was obtained from the University of Alabama at Birmingham Institutional Review Board (IRB-150910007) to conduct the current study. The need for consent was waived by the institutional review board from the University of Alabama at Birmingham.

Consent for publication

No individual patient data is presented in this study and thus consent to publish is not applicable.

Competing interests

The authors declare that they have no competing interests.

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Authors’ Affiliations

(1)
Department of Medicine, Division of Allergy, Pulmonary and Critical Care Medicine, 1900 University Blvd, THT 422, Birmingham, AL 35294, USA
(2)
Gregory Fleming James Cystic Fibrosis Research Center, Birmingham, AL, USA

References

  1. Rommens JM, Iannuzzi MC, Kerem B, Drumm ML, Melmer G, Dean M, Rozmahel R, Cole JL, Kennedy D, Hidaka N, et al. Identification of the cystic fibrosis gene: chromosome walking and jumping. Science. 1989;245:1059–65.View ArticlePubMedGoogle Scholar
  2. Balough K, McCubbin M, Weinberger M, Smits W, Ahrens R, Fick R. The relationship between infection and inflammation in the early stages of lung disease from cystic fibrosis. Pediatr Pulmonol. 1995;20:63–70.View ArticlePubMedGoogle Scholar
  3. Ogrinc G, Kampalath B, Tomashefski JF Jr. Destruction and loss of bronchial cartilage in cystic fibrosis. Hum Pathol. 1998;29:65–73.View ArticlePubMedGoogle Scholar
  4. Kosorok MR, Zeng L, West SE, Rock MJ, Splaingard ML, Laxova A, Green CG, Collins J, Farrell PM. Acceleration of lung disease in children with cystic fibrosis after pseudomonas aeruginosa acquisition. Pediatr Pulmonol. 2001;32:277–87.View ArticlePubMedGoogle Scholar
  5. Kerem E, Viviani L, Zolin A, MacNeill S, Hatziagorou E, Ellemunter H, Drevinek P, Gulmans V, Krivec U, Olesen H, Group EPRS. Factors associated with fev1 decline in cystic fibrosis: analysis of the ecfs patient registry. Eur Respir J. 2014;43:125–33.View ArticlePubMedGoogle Scholar
  6. Taccetti G, Campana S, Festini F, Mascherini M, Doring G. Early eradication therapy against pseudomonas aeruginosa in cystic fibrosis patients. Eur Respir J. 2005;26:458–61.View ArticlePubMedGoogle Scholar
  7. Proesmans M, Vermeulen F, Boulanger L, Verhaegen J, De Boeck K. Comparison of two treatment regimens for eradication of pseudomonas aeruginosa infection in children with cystic fibrosis. J Cyst Fibros. 2013;12:29–34.View ArticlePubMedGoogle Scholar
  8. Mayer-Hamblett N, Kloster M, Rosenfeld M, Gibson RL, Retsch-Bogart GZ, Emerson J, Thompson V, Ramsey BW. Impact of sustained eradication of new pseudomonas aeruginosa infection on long-term outcomes in cystic fibrosis. Clin Infect Dis. 2015;61:707–15.View ArticlePubMedPubMed CentralGoogle Scholar
  9. Gibson RL, Burns JL, Ramsey BW. Pathophysiology and management of pulmonary infections in cystic fibrosis. Am J Respir Crit Care Med. 2003;168:918–51.View ArticlePubMedGoogle Scholar
  10. Frangolias DD, Mahenthiralingam E, Rae S, Raboud JM, Davidson AG, Wittmann R, Wilcox PG. Burkholderia cepacia in cystic fibrosis. Variable disease course. Am J Respir Crit Care Med. 1999;160:1572–7.View ArticlePubMedGoogle Scholar
  11. Courtney JM, Dunbar KE, McDowell A, Moore JE, Warke TJ, Stevenson M, Elborn JS. Clinical outcome of burkholderia cepacia complex infection in cystic fibrosis adults. J Cyst Fibros. 2004;3:93–8.View ArticlePubMedGoogle Scholar
  12. Ledson MJ, Gallagher MJ, Jackson M, Hart CA, Walshaw MJ. Outcome of burkholderia cepacia colonisation in an adult cystic fibrosis centre. Thorax. 2002;57:142–5.View ArticlePubMedPubMed CentralGoogle Scholar
  13. Horsley A, Webb K, Bright-Thomas R, Govan J, Jones A. Can early burkholderia cepacia complex infection in cystic fibrosis be eradicated with antibiotic therapy? Front Cell Infect Microbiol. 2011;1:18.View ArticlePubMedPubMed CentralGoogle Scholar
  14. Regan KH, Bhatt J. Eradication therapy for burkholderia cepacia complex in people with cystic fibrosis. Cochrane Database Syst Rev. 2014;10:CD009876.Google Scholar
  15. Flume PA, Mogayzel PJ Jr, Robinson KA, Goss CH, Rosenblatt RL, Kuhn RJ, Marshall BC. Clinical practice guidelines for pulmonary therapies C. Cystic fibrosis pulmonary guidelines: treatment of pulmonary exacerbations. Am J Respir Crit Care Med. 2009;180:802–8.View ArticlePubMedGoogle Scholar
  16. Ramsay KA, Butler CA, Paynter S, Ware RS, Kidd TJ, Wainwright CE, Bell SC. Factors influencing acquisition of burkholderia cepacia complex organisms in patients with cystic fibrosis. J Clin Microbiol. 2013;51:3975–80.View ArticlePubMedPubMed CentralGoogle Scholar
  17. St Denis M, Ramotar K, Vandemheen K, Tullis E, Ferris W, Chan F, Lee C, Slinger R, Aaron SD. Infection with burkholderia cepacia complex bacteria and pulmonary exacerbations of cystic fibrosis. Chest. 2007;131:1188–96.View ArticlePubMedGoogle Scholar
  18. Kitt H, Lenney W, Gilchrist FJ. Two case reports of the successful eradication of new isolates of burkholderia cepacia complex in children with cystic fibrosis. BMC Pharmacol Toxicol. 2016;17:14.View ArticlePubMedPubMed CentralGoogle Scholar
  19. Ball R, Brownlee KG, Duff AJ, Denton M, Conway SP, Lee TW. Can burkholderia cepacia complex be eradicated with nebulised amiloride and tobi? J Cyst Fibros. 2010;9:73–4.View ArticlePubMedGoogle Scholar
  20. Middleton PG, Kidd TJ, Williams B. Combination aerosol therapy to treat burkholderia cepacia complex. Eur Respir J. 2005;26:305–8.View ArticlePubMedGoogle Scholar

Copyright

© The Author(s). 2018

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