Skip to main content

Invasive pulmonary aspergillosis in immunocompetent patients hospitalised with influenza A-related pneumonia: a multicenter retrospective study

Abstract

Background

Increasing cases of pulmonary aspergillosis (IPA) in immunocompetent patients with severe influenza have been reported. Howevere, the risk factors for occurence and death are largely unknown.

Methods

Data of hospitalised patients with influenza A-related pneumonia (FluA-p) obtained from five teaching hospitals from 2031 to 2018, were reviewed. Univariate and multivariate logistical regression analyses were performed to determine the risk factors involved in the acquisition and 60-day mortality in IPA patients.

Results

Of the 693 FluA-p patients included in the study, 3.0% (21/693) were IPA patients with a 60-day mortality of 42.9% (9/21). Adjusted for confounders, a Cox proportional hazard model showed that IPA was associated with increased risk for 60-day mortality [hazard ratio (HR) 4.336, 95% confidence interval (CI) 1.191–15.784, p = 0.026] in FluA-p patients. A multivariate logistic regression model confirmed that age (odd ratio (OR) 1.147, 95% CI 1.048–1.225, p = 0.003), systemic corticosteroids use before IPA diagnosis (OR 33.773, 95% CI 5.681–76.764, p <  0.001), leukocytes > 10 × 109/L (OR 1.988, 95% CI 1.028–6.454, p = 0.029) and lymphocytes < 0.8 × 109/L on admission (OR 34.813, 95% CI 1.676–73.006, p = 0.022), were related with the acquisition of IPA. Early neuraminidase inhibitor use (OR 0.290, 95% CI 0.002–0.584, p = 0.021) was associated with a decreased risk for a 60-day mortality in IPA patients.

Conclusions

Our results showed that IPA worsen the clinical outcomes of FluA-p patients. The risk factors for the acquisition and death were helpful for the clinicians in preventing and treating IPA.

Peer Review reports

Background

Influenza is a respiratory infectious disease, caused by influenza viruses, and which can present seasonal epidemics and pandemics [1, 2]. Despite the progress in medical technologies and economic development, influenza still causes considerate complications and mortality [3]. Following infection by influenza viruses, patients can show a broad spectrum of clinical symptoms, ranging from self-limited upper respiratory tract illness to severe pneumonia and acute respiratory distress syndrome (ARDS) [4]. It was estimated that every year, 10–20% of the global population experienced symptomatic influenza, including 3–5 million severe illnesses and 260–640 thousand deaths [5].

Nearly half of severe influenza patients present with pneumonia, which is mostly caused by influenza A [6]. Influenza pneumonia is often coinfected with other pathogens and this worsen the clinical symptoms and deteriorates the outcomes [7, 8]. Previous studies found, that Streptococcus pneumoniae, Staphylococcus aureus and Haemophilus influenzae, were the most common etiologies in coinfected influenza [9]. The Chinese and American guidelines recommend empiric antibiotics use to fight the pathogens associated with severe influenza [10, 11]. Invasive pulmonary aspergillosis mostly and traditionally occurs in immunocompromised hosts, such as patients with hematopoietic stem cell transplantation, granulocyte deficiency and organ transplant recipients; but rarely in those with normal immune function [12, 13]. In recent years, more cases of IPA have been reported in severe influenza patients and with increased mortality [14,15,16]. The most notable was that over 30% of these cases had no classic immunocompromised factors.

However, there are limited data on influenza-associated pulmonary aspergillosis (IAPA), especially in prior immunocompetent patients. The incidence and disease characteristics were largely unknown. Identifying those patients with high risk for IPA, remains challenging. We carried out this multicenter retrospective study with the following purposes: i) to investigate the incidence and risk factors for IPA acquisition in immunocompetent, adult patients hospitalised with influenza A-related pneumonia (FluA-p); ii) to explore the risk factors associated with a 60-day mortality in IAPA patients.

Methodology

Study design and population

We screened hospitalised patients for positive influenza A virus RNA using respiratory specimens by reverse transcription polymerase chain reaction (RT-PCR) from microbiology laboratories of five teaching hospitals in Beijing, Shandong and Yunnan Provinces and during the period from 1st January to 31st December 2018 (the details of the five centers are shown in Additional file 1: Appendix file 1). From this data, we retrieved all cases had both influenza and radiograph proven pneumonia.

Patients were excluded if [17] (1) age < 18 years; (2) pneumonia onset ≥48 h after admission and not been hospitalised within the last 28 days, because the consensus of nosocomial pneumonia caused by influenza was difficult; (3) Immunocompromised status [18].

Group division

We divided the patients into two groups: the case group included patients with FluA-p, who subsequently became infected with proven and/or probable invasive pulmonary aspergillosis (IPA group); and the control group that included patients with FluA-p and who showed no evidence of Aspergillus infection while hospitalised.

Study definitions

Patients with FluA-p were defined as patients with respiratory symptoms and a new pulmonary infiltrate on the chest radiograph, combined with positive influenza virus A RT-PCR testing during the influenza seasons.

A Proven IPA was defined as the microscopic evidence of dichotomous branching hyphae with a positive culture for Aspergillus through an endobronchial biopsy, irrespective of host factors or clinical features [18].

According to the revised definitions of invasive fungal diseases from the European Organization for Research and Treatment of Cancer/Invasive Fungal Infections Cooperative Group and the National Institute of Allergy and Infectious Diseases Mycoses Study Group (EORTC/MSG) in 2019 [18], a probable IPA required a host factor, clinical features and mycological evidence of aspergillosis. However, these criteria were created for immunosuppressed hosts and influenza-related aspergillosis may occur in previously normal hosts. Thus, the host factors were not required in our study. The clinical features included one of the following signs or symptoms: refractory fever to at least 3 days of appropriate antibiotic treatment; recrudescent fever after a period of defervescence of at least 48 h, while still on antibiotics and without other apparent cause; dyspnea; hemoptysis; pleural friction rub or chest pain; worsening respiratory insufficiency in spite of appropriate antibiotic therapy and ventilatory support. The radiological criteria included any infiltrates on pulmonary imaging detected by chest x-ray or CT scan of the lungs. The mycological evidence included: a positive Aspergillus culture from a bronchoalveolar lavage (BAL); a galactomannan (GM) optical index on BAL of ≥1.0; a GM optical index on serum of ≥0.5 [18].

Early neuraminidase inhibitor (NAI) treatment was defined as any NAI (e.g., oseltamivir, zanamivir and peramivir) administered within 2 days after disease onset [10, 11]. The coinfection with other Aspergillus pathogens was defined by community-acquired respiratory co-pathogens that was identified within 2 days of hospital admission [19]. The conditions of a community-acquired respiratory co-pathogen was defined as the definite or probable etiology (Additional file 1: Appendix 2). Immunocompetent hosts were defined as patients without immunocompromised factors described above.

Data collection

Data was retrospectively collected and included demographic information, underlying disease (Additional file 1: Appendix 3), clinical manifestations, laboratory and radiological findings, microbiologic diagnosis, treatment (use of antiviral agents, corticosteroids, vasopressors and mechanical ventilation), clinical outcomes (complications during hospitalisation, admittance to the intensive care unit (ICU) and a 60-day mortality).

Statistical analysis

All data were analyzed with SPSS 22.0 and measurement data were tested for normality by Kolmogorov-Smirnov. The measurement data of normal distributions were reported as mean ± standard deviation. Measurements data of non-normal distributions were reported as median. The categorical variables were analyzed by the Chi-square test or Fisher’s exact test, and continuous variables were analyzed by the student t-test or the Mann–Whitney U-test. A p-value of < 0.05 was considered statistically significant and all probabilities were two-tailed. A Cox proportional hazard model was performed to evaluate the effect of IPA on a 60-day mortality in FluA-p patients. The model was adjusted by age, gender, comorbidities, leukocytes > 10 × 109/L, serum procalcitonin > 0.1 ng/ml, coinfection with non-Aspergillus pathogens and early use of neuraminidase inhibitors. The clinical characteristics were compared between patients in the IPA and control groups (Table 1). Variables with p-values of ≤0.05 on univariate analysis were subsequently entered into the backward stepwise logistic regression analysis to identify risk factors for the acquisition and death in IPA patients.

Table 1 Demographic and clinical characteristics between the two groups

Results

Screening process

We screened 2187 hospitalised patients with positive influenza A RNA. Overall, 693 immunocompetent adult patients hospitalised with FluA-p onset in the community were entered into the final analysis. The proportion of patients who developed IPA during hospitalisation was 3.0% (21/693) (Fig. 1).

Fig. 1
figure1

Patient screening algorithm for FluA-p

Overview of patients with FluA-p

Overall, the median age was 61.0 years old and the male accounted for 66.5% (461/693). Fifty-eight percent of patients (402/693) had at least one underlying disease with the top three being diabetes mellitus 13.3% (92/693), cerebrovascular disease 10.4% (72/693) and chronic obstructive pulmonary disease 5.8% (40/693). The proportion of patients with PO2/FiO2 < 300 mmHg was 53.2% (340/639). Cavities and multiple nodules in chest radiology were seen in 2.7% (19/693) and 21.8% (151/693) of patients, respectively.

Nineteen percent (132/693) of FluA-p patients used a systemic dose of 0.6 ± 0.3 mg/kg corticosteroids before IPA diagnosis. All patients were administrated with NAI during the disease course, while 33.3% (231/693) received NAIs within the 48 h after illness onset. 24.1% (167/693) of patients had complications with respiratory failure, 21.2% (147/693) had heart failure, 5.2% (36/693) had septic shocks during hospitalisation, 26.3% (182/693) of patients were admitted to intensive care unit (ICU) and the 60-day mortality was 20.9% (145/693) (Table 1).

Comparisons between the IPA and control patients

Compared with control patients, the IPA patients were older (67.0 yrs. vs. 60.0 yrs., p <  0.001), had more frequency of diabetic (71.4% vs. 11.5%, p <  0.001), chronic pulmonary disease (28.6% vs 5.1%, p <  0.001), asthma (14.3% vs. 2.4%, p = 0.009) and chronic heart failure (14.3% vs. 0.0%, p <  0.001), and lower levels of body mass index (BMI) [(21.5 ± 0.4) kg/m2 vs. (24.5 ± 3.6) kg/m2, p = 0.014]. The proportion of leukocytes > 10 × 109/L (71.4% vs. 15.3%, p <  0.001), lymphocytes < 0.8 × 109/L (71.4% vs. 43.3%, p = 0.011), albumin < 35 g/L (28.6% vs. 8.4%, p = 0.006), PO2/FiO2 < 300 mmHg (76.2% vs 52.4%, p = 0.032) and radiologic cavities (14.3% vs. 2.4%, p = 0.009), were significantly higher in the IPA patients; while, serum procalcitonin (PCT) >  0.1 ng/ml (9.5% vs. 47.3%, p = 0.001) was more common in the control patients. More IPA patients used corticosteroids inhalers (14.3% vs. 0.0%, p <  0.001) and systemic corticosteroids (85.7% vs. 17.0%, p <  0.001) before IPA diagnosis. However, no significant differences in the dose of systemic corticosteroids was observed.

Complications of respiratory failure (100.0% vs. 21.7%, p <  0.001), heart failure (42.9% vs. 20.5%, p = 0.028) and septic shock (85.7% vs. 2.7%, p <  0.001) were more frequent in IPA patients. The proportion of patients needing noninvasive ventilation (42.9% vs. 22.3%, p = 0.014), invasive ventilation (85.7% vs. 20.8%, p <  0.001) and vasopressor use (52.4% vs. 2.4%, p <  0.001), were higher in IPA patients. More IPA patients were admitted to ICU (71.4% vs. 24.9%, p <  0.001) and had a higher 60-day mortality rate (42.9% vs. 18.9%, p = 0.015) (Table 2).

Table 2 Supportive treatments and clinical outcomes between the two groups

Diagnosis of IPA

The mean duration from the diagnosis of IPA to the day of admission was 6.4 ± 4.8 days, with a range of 2–18 days. A serum GM test was performed in 15 of the 21 IPA patients. Seventeen IPA patients were subjected to a GM test in BAL. Fourteen patients had a GM optical index on BAL of ≥1.0, five patients a GM optical index of single serum of ≥0.5.

In all 21 IPA patients, a BAL culture was performed that led to the identification of Aspergillus in 6 patients’ cultures. Two patients were diagnosed as proven IPA by trans-bronchial lung biopsy (both were Aspergillus fumigatus). A probable IPA diagnosis was performed in 19 of the 21 IPA patients (Table 3).

Table 3 Diagnosis of IPA

Coinfection with non-Aspergillus pathogens isolated in FluA-p patients

Coinfection with other community-acquired pathogens was diagnosed in only 3 of 21 IPA patients and 1 patient was diagnosed with S. pneumoniae, 1 patient with P. aeruginosa and 1 with K. pneumoniae. While, 39.0% (262/672) of control patients were diagnosed with non-Aspergillus etiologies, S. pneumoniae was the most common diagnosed pathogen with 33.2% (87/262), followed by K. pneumoniae 30.5% (80/262) and Staphylococcus aureus 20.6% (54/262) (Additional file 1: Appendix file 4).

Effect of IPA on the 60-day mortality of FluA-p patients

Adjusted for age, gender, comorbidities, blood leukocyte counts > 10 × 109/L, serum PCT >  0.1 ng/ml, coinfection with other pathogens and early NAIs use, a Cox proportional hazard model showed that IPA was associated with an increased risk in the 60-day mortality of FluA-p patients [hazard ratio (HR) 4.336, 95% confidence interval (CI) 1.191–15.784, p = 0.026) (Table 4).

Table 4 The impact of IPA on the 60-day mortality in FluA-p patients

Kaplan-Meier survival curve showed that the 60-day mortality of the IPA patients was significantly higher than that of the control patients (p = 0.006 for the log rank test) (Fig. 2).

Fig. 2
figure2

Kaplan-Meier survival graph for FluA-p patients with and without IPA (censored at 60d)

Risk factors associated with IPA occurrence in FluA-p patients

To explore the risk factors for IPA acquisition, the following variables were entered into the backstep logistic regression model: age, BMI, diabetes mellitus, asthma, chronic congestive heart failure, leukocytes > 10 × 109/L, lymphocytes < 0.8 × 109/L, albumin < 35 g/L, serum PCT >  0.1 ng/ml, cavity on chest radiology, use of inhaled corticosteroids and systemic corticosteroids before IPA diagnosis, and coinfection with other community-acquired pathogens, and the analyses led to the following results: age (OR 1.147, 95% CI 1.048–1.225, p = 0.003), systemic corticosteroids use before IPA diagnosis (OR 33.773, 95% CI 5.681–76.764, p <  0.001), leukocytes > 10 × 109/L (OR 1.988, 95% CI 1.028–6.454, p = 0.029) and lymphocytes < 0.8 × 109/L on admission (OR 34.813, 95% CI 1.676–73.006, p = 0.022). These results were proven to be independently related to the IPA acquisition in FluA-p patients (Table 5).

Table 5 Predictors for acquisition and 60-day mortality of IPA in FluA-p patients

Predictors for a 60-day mortality of IPA in FluA-p patients

The demographic features and comorbidities were similar between the survival and deceased patients with IPA. Of the 21 patients that received an antifungal treatment within 24 h after IPA diagnosis, 18 patients used voriconazole and 3 patients used a combination treatment (voriconazole + echinocandin). However, no significant difference was found in antifungal therapy between the two groups. Compared with the survival group, the deceased patients’ group had a higher proportion of lymphocytes < 0.8 × 109/L (100.0% vs 50.0%, p = 0.043) and lower proportion of early NAIs use (11.1% vs 75.0%, p = 0.014) (Additional file 1: Appendix file 5).

A multivariate logistic regression model confirmed early NAIs use (OR 0.290, 95% CI 0.002–0.584, p = 0.021) and that was the only predictor for the 60-day mortality in IPA patients (Table 5).

Discussion

Our study has two important findings: 1) the prevalence of IPA in immunocompetent adult patients hospitalised with FluA-p, was 3.0%. However, it was associated with increased mortality; 2) we identified age, leukocytes, lymphocytes and systemic corticosteroids use as risk factors for IPA diagnosis. Early NAIs use was related to better outcomes, which were helpful in the prevention and treatment of IPA patients with severe influenza.

There are rare data on the incidence of IPA among all hospitalised FluA-p patients and previous studies were limited to patients admitted to ICU. In our study, the incidence of IPA in ICU patients was 8.2%, which was consistent with the 7.2–8.8% reported by Rice [20] and Martin-Loeches [21]; but, this was lower than the reported 19.2% in the Schauwvlieghe’s study [22]. Further analysis revealed that all IPA patients had respiratory failure complications and an IPA incidence of 12.6%. This observation is very close to that of the Schauwvlieghe’s study, in which the IPA incidence was 14.2% in non-immunocompromised severe influenza patients. The difference in reported IPA incidences could be explained by the severity of influenza illness, the detection capability of IPA and the discrepancy in ICU admission criteria in the different medical systems.

Although the IPA incidence in our study was not high, the 60-day mortality was as high as 42.9%, which was in accordance with previous reports of 33–71% [12, 14,15,16, 19, 22,23,24]. In addition to the genetic background, there were at least two reasons for the difference in mortality among those studies: 1) the influence of the patients’ immunity status before influenza onset. In the Schauwvlieghe’s study [22], the 90-day mortality in immunocompetent patients with IPA, was 33%; while, it was 71% in patients with immunocompromised factors. Among Huang’s research population [24], 24% received immunosuppressive agents and the overall ICU mortality was 41.3%. All the patients in our study were previously immunocompetent and the mortality was lower than that in the previously reported immunocompromised patients’ studies. 2) the outcomes were profoundly affected by the awareness, timing and approach of pathogenic testing that were performed for invasive aspergillosis, leading to a delayed IPA diagnosis and treatment. For example, a center in the Schauwvlieghe’s [22] study paid more attention to IPA because of several previous reports. As a result, the diagnostic and survival rate of IPA were much higher than that in other centers. Adjusted by age, sex, comorbidities, early use of NAIs and probable co-infection with other etiologies, a Cox proportional hazard model showed, that IPA independently increases the risk for 60-day mortality in FluA-p patients, by 3 times. Our results implied the importance of IPA screening in patients with severe influenza pneumonia, regardless of their previous immunity status. This allows an early diagnosis in patients, that prompts carrying out an antifungal treatment, as soon as possible.

In our study, IPA patients were older in age and with frequent comorbidities. Diabetes and chronic airway diseases (such as COPD and asthma) were the most common underlying diseases. Hypoproteinemia was common, suggesting that their nutritional statuses were poor. When the radiological findings of IPA patients were reviewed, it showed mainly pulmonary infiltrates, with a slightly higher proportion of cavities than the control patients; but, no obvious halo sign was found. Previous studies also showed that the halo sign was only seen in less than 5% of patients with secondary IPA influenza [25, 26]. The multivariate logistic regression analysis confirmed, that age, increased leukocyte counts, decreased lymphocyte counts and systemic corticosteroids use, were independent risk factors for the acquisition of IPA in immunocompetent patients hospitalised with FluA-p. It is believed that the pathogenesis of invasive aspergillosis, in the setting of influenza infection, may be due to both local and systemic effects of the virus. Local effects include influenza and inflammation damage of the bronchoalveolar epithelial cells, that lead to the impairment of the barrier function and dysfunction of ciliary motility and clearance [27]. Systemically, influenza alters the Th1/Th2 balance and causes lymphopenia. The immune function of the elderly patients significantly decreased, and their resistance to infection was poor. Previous studies also showed that elderly influenza patients, were more vulnerable to secondary infections [28]. Elevated leukocyte counts in influenza pneumonia patients is usually associated with bacterial or fungal coinfection; while, serum PCT is a relatively specific biomarker of bacterial infection [29]. In our study, more than 90% of IPA patients had a serum PCT of < 0.1 ng/ml, indicating that the coinfection with bacteria was not common. Therefore, the elevated leukocytes with normal serum PCT level, strongly suggested fungal infection. Lymphocytes reflected the function of cellar immunity, which was the main anti-viral mechanism in humans. The suppression of cellar immunity delays the clearance of the virus, along with a more serious damage of airway, thus creating conditions for an invasive Aspergillus infection.

The steroids are the most common immunomodulators in clinic, with powerful depressive effects on both cellar immunity and humoral immunity. Corticosteroids use often causes secondary fungal infection [30]. Our study confirmed the association of systemic corticosteroids use and occurrence of IPA as previously reported [22, 24]. Meanwhile, we found, that even a low-to-moderate dose and short-term systemic corticosteroids use, would increase the risk for of IPA acquisition in the predisposition to immune suppression, caused by severe influenza. Traditionally, it was believed that a prolonged use of steroids (at least 3 weeks and a prednisone equivalent of > 0.3 mg/kg/d) was related to IPA occurrence [31]. It should be noted that there may be overuse of systemic steroids in influenza patients. In numerous reports, more than 50% of influenza patients received systemic corticosteroids, which were proven to be associated with poor outcomes [32, 33]. In our study, 19% of FluA-p patients received systemic steroids during hospitalisation. Although in severe influenza, uncontrolled immune response is involved in organic damage and increased mortality. In animal models, corticosteroid treatment was found to decrease mortality and ameliorate acute lung injury induced by influenza [34]. By now, except for septic shock patients with vasopressor-dependence or adrenal insufficiency, there is no consensus on steroids use in the treatment of severe influenza. Our study confirmed, that the systemic steroids use, was associated with increased risk for IPA and with an unacceptable high mortality, and even in prior immunocompetent FluA-p patients. Therefore, we thought that it was urgent to regulate the use of systemic corticosteroids in the influenza setting.

In our study, early NAIs use was proven to be the only predictor associated with better outcomes in IAPA patients, by decreasing the risk of more than 70% for a 60-day mortality. Previous studies also proved that the sooner NAIs were used, the better were the outcomes in severe influenza patients [35, 36]. According to the mechanism of IAPA, it is reasonable that early inhibition of viral duplication and alleviation of lung damage, caused by virus and inflammation, can create favorable conditions for controlling the occurrence and development of IAPA. However, why early use of NAIs had not shown a decrease in the risk for IAPA incidence, was still unclear. Apart from the small size of the population, our study implies that there is a complex pathogenesis for IPA occurrence in severe influenza patients.

As far as we know, this was the only investigation focused on IPA in immunocompetent patients hospitalised with FluA-p. Unlike other studies that were limited to ICU patients, our study included, not only patients in ICU, but also patients from general wards. IPA patients, that were not admitted to ICU in other studies and for some reasons, could be included into our population; which, reduced the selective bias. In addition, the population in our study had no classic immunocompromised factors. The occurrence of IPA in these patients was most likely to be neglected. Therefore, the results of our study provide a great significance for clinicians as it allows to identify patients with increased risk for IPA acquisition and at an early stage, advocating therefore, a prompt prevention and treatment.

There were some limitations specific to our study: 1) Besides the nature of the retrospective study, the relatively small sample size along with some missing data, might limit the accuracy of the results; 2) Though the latest diagnostic criteria of IPA was used, the proportion of the microbiologic examinations for IPA in the control patients, was low. Especially, BAL samples were only performed in less than 25%; while, a serum GM testing was performed in 47% of control patients. Some studies showed, that with the current diagnostic standard of serum GM optical index (OD) >  0.5, nearly half of IPA cases would be under-detected [37, 38]. In addition, the triggers to perform BAL were not clarified due to the restrospective study design, and IPA complicating influenza might develope post-admission. This may have limited the number of true cases found and caused selective bias. Therefore, the actual IPA incidence in our study might be under-detected; 3) more than 1/3 of the patients had not performed influenza subtype testing and other respiratory tract viruses were not routinely detected. Thus, we could not exclude coinfection with other viruses.

Conclusions

Our study showed that there is approximatively 3.0% of IPA incidence with an increased mortality that was observed even in immunocompetent patients, hospitalised with FluA-p. Additionally, we identified age, elevated leukocytes, reduced lymphocytes, on the day of admission and systemic corticosteroids use, as risk factors for IPA acquisition, and that early NAIs use was a predictor of better outcome. Meanwhile, it is suggested that these results should be confirmed using prospective and large sample studies to further verify these conclusions.

Availability of data and materials

All data generated or analysed during this study are included in this published article and its supplementary information files.

Abbreviations

Flu-p:

Influenza-related pneumonia

IPA:

Invasive pulmonary aspergillosis

NAI:

Neuraminidase inhibitor

OR:

Odds ratio

HR:

Hazard ratio

95% IC :

95% Interval confidence

FluA-p:

Influenza A-related pneumonia

RT-PCR:

Reverse transcription polymerase chain reaction

EORTC/MSG:

European Organization for Research and Treatment of Cancer/Invasive Fungal Infections Cooperative Group and the National Institute of Allergy and Infectious Diseases Mycoses Study Group

BAL:

Bronchoalveolar lavage

GM:

Galactomannan

IQR:

Interquartile range

BMI:

Body mass index

COPD:

Chronic obstructive pulmonary disease

SBP:

Systolic blood pressure

Hb:

Hemoglobin

BG:

Blood glucose

ALB:

Albumin

BUN:

Blood urea nitrogen

PH:

Hydrogen ion index

pO2/FiO2 :

Arterial pressure of oxygen/fraction of inspiration oxygen

ICs:

Inhaled corticosteroid

PCT:

Procalcitonin

ICU:

Intensive care unit

References

  1. 1.

    Peteranderl C, Herold S, Schmoldt C. Human influenza virus infections. Semin Respir Crit Care Med. 2006;37(4):487–500.

    Article  Google Scholar 

  2. 2.

    Neuzil KM, Bresee JS, de la Hoz F, Johansen K, Karron RA, Krishnan A, et al. Data and product needs for influenza immunization programs in low- and middle-income countries: rationale and main conclusions of the WHO preferred product characteristics for next-generation influenza vaccines. Vaccine. 2017;35(43):5734–7.

    PubMed  Article  Google Scholar 

  3. 3.

    Dahal S, Jenner M, Dinh L, Mizumoto K, Viboud C, Chowell G. Excess mortality patterns during 1918-1921 influenza pandemic in the state of Arizona, USA. Ann Epidemiol. 2016;28(5):273–80.

    Article  Google Scholar 

  4. 4.

    Kumar V. Influenza in Children. Indian J Pediatr. 2017;84(2):139–43.

    PubMed  Article  Google Scholar 

  5. 5.

    Lozano R, Naghavi M, Foreman K, Lim S, Shibuya K, Aboyans V, et al. Global and regional mortality from 235 causes of death for 20 age groups in 1990 and 2010: a systematic analfor the global burden of disease study 2010. Lancet. 2012;380(9859):2095–128.

    PubMed  Article  Google Scholar 

  6. 6.

    Li M, Bolker BM, Dushoff J, Ma J, Earn DJD. Patterns of seasonal and pandemic influenza-associated health care and mortality in Ontario, Canada. BMC Public Health. 2019;19(1):1237.

    PubMed  PubMed Central  Article  Google Scholar 

  7. 7.

    Klein EY, Monteforte B, Gupta A, Jiang W, May L, Hsieh YH, Dugas A. The frequency of influenza and bacterial coinfection: a systematic review and meta-analysis. Influenza Other Respir Viruses. 2016;10(5):394–403.

    PubMed  PubMed Central  Article  Google Scholar 

  8. 8.

    Kylla H, Dutta TK, Roychoudhury P, Subudhi PK. Coinfection of diarrheagenic bacterial and viral pathogens in piglets of northeast region of India. Vet World. 2019;12(2):224–30.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  9. 9.

    Brundage JF. Interactions between influenza and bacterial respiratory pathogens: implications for pandemic preparedness. Lancet Infect Dis. 2016;6(5):303–12.

    Article  Google Scholar 

  10. 10.

    Harper SA, Bradley JS, Englund JA, File TM, Gravenstein S, Hayden FG, et al. Seasonal influenza in adults and children--diagnosis, treatment, chemoprophylaxis, and institutional outbreak management: clinical practice guidelines of the Infectious Diseases Society of America. Clin Infect Dis. 2019;48(8):1003–32.

    Article  Google Scholar 

  11. 11.

    Cao B, Huang Y, She DY, Cheng QJ, Fan H, Tian XL, et al. Diagnosis and treatment of community-acquired pneumonia in adults: 2016 clinical practice guidelines by the Chinese thoracic society, Chinese Medical Association. Clin Respir J. 2018;12(4):1320–60.

    PubMed  Article  Google Scholar 

  12. 12.

    Cadena J, Thompson GR 3rd, Patterson TF. Invasive Aspergillosis: current strategies for diagnosis and management. Infect Dis Clin N Am. 2016;30(1):125–42.

    Article  Google Scholar 

  13. 13.

    Solano C, Vázquez L. Invasive aspergillosis in the patient with oncohematologic disease. Rev Iberoam Micol. 2018;35(4):198–205.

    PubMed  Article  Google Scholar 

  14. 14.

    Alshabani K, Haq A, Miyakawa R, Palla M, Soubani AO. Invasive pulmonary aspergillosis in patients with influenza infection: report of two cases and systematic review of the literature. Expert Rev Respir Med. 2015;9(1):89–96.

    CAS  PubMed  Article  Google Scholar 

  15. 15.

    Kim SH, Kim MN, Lee SO, Choi SH, Kim YS, Woo JH, et al. Fatal pandemic influenza A/H1N1 infection complicated by probable invasive pulmonary aspergillosis. Mycoses. 2012;55(2):189–92.

    PubMed  Google Scholar 

  16. 16.

    Lat A, Bhadelia N, Miko B, Furuya EY, Thompson GR 3rd. Invasive aspergillosis after pandemic (H1N1) 2009. Emerg Infect Dis. 2010;16(6):971–3.

    PubMed  PubMed Central  Article  Google Scholar 

  17. 17.

    Chen L, Zhou F, Li H, Xing X, Han X, Wang Y, et al. Disease characteristics and management of hospitalised adolescents and adults with community-acquired pneumonia in China: a retrospective multicentre survey. BMJ Open. 2018;8(2):e018709.

    PubMed  PubMed Central  Article  Google Scholar 

  18. 18.

    Donnelly JP, Chen SC, Kauffman CA, Steinbach WJ, Baddley JW, Verweij PE, et al. Revision and Update of the Consensus Definitions of Invasive Fungal Disease From the European Organization for Research and Treatment of Cancer and the Mycoses Study Group Education and Research Consortium. Clin Infect Dis. 2019;ciz1008.

  19. 19.

    Ku YH, Chan KS, Yang CC, Tan CK, Chuang YC, Yu WL, et al. Higher mortality of severe influenza patients with probable aspergillosis than those with and without other coinfections. J Formos Med Assoc. 2017;116(9):660–70.

    PubMed  Article  Google Scholar 

  20. 20.

    Rice TW, Rubinson L, Uyeki TM, Vaughn FL, John BB, Miller RR 3rd, et al. Critical illness from 2009 pandemic influenza A virus and bacterial coinfection in the United States. Crit Care Med. 2012;40(5):1487–98.

    PubMed  PubMed Central  Article  Google Scholar 

  21. 21.

    Martin-Loeches I, Schultz JM, Vincent JL, Alvarez-Lerma F, Bos LD, Solé-Violán J, et al. Increased incidence of co-infection in critically ill patients with influenza. Intensive Care Med. 2017;43(1):48–58.

    PubMed  Article  Google Scholar 

  22. 22.

    Schauwvlieghe AFAD, Rijnders BJA, Philips N, Verwijs R, Vanderbeke L, Van Tienen C, et al. Invasive aspergillosis in patients admitted to the intensive care unit with severe influenza: a retrospective cohort study. Lancet Respir Med. 2018;6(10):782–92.

    PubMed  Article  Google Scholar 

  23. 23.

    Cattaneo C, Gramegna D, Malagola M, Pagani C, Borlenghi E, Cerqui E, et al. Invasive pulmonary aspergillosis in acute leukemia: a still frequent condition with a negative impact on the overall treatment outcome. Leuk Lymphoma. 2019;60(12):3044–50.

    PubMed  Article  Google Scholar 

  24. 24.

    Huang L, Zhang N, Huang X, Xiong S, Feng Y, Zhang Y, et al. Invasive pulmonary aspergillosis in patients with influenza infection: A retrospective study and review of the literature. Clin Respir J. 2019;13(4):202–11.

    CAS  PubMed  Article  Google Scholar 

  25. 25.

    Vandewoude KH, Blot SI, Depuydt P, Benoit D, Temmerman W, Colardyn F, Vogelaers D. Clinical relevance of Aspergillus isolation from respiratory tract samples in critically ill patients. Crit Care. 2006;10(1):R31.

    PubMed  PubMed Central  Article  Google Scholar 

  26. 26.

    Park SY, Lim C, Lee SO, Choi SH, Kim YS, et al. Computed tomography findings in invasive pulmonary aspergillosis in non-neutropenic transplant recipients and neutropenic patients, and their prognostic value. J Inf Secur. 2011;63(6):447–56.

    Google Scholar 

  27. 27.

    Crum-Cianflone NF. Invasive Aspergillosis Associated With Severe Influenza Infections. Open Forum Infect Dis. 2016;3(3):ofw171.

    PubMed  PubMed Central  Article  Google Scholar 

  28. 28.

    Rosano A, Bella A, Gesualdo F, Acampora A, Pezzotti P, Marchetti S, et al. Investigating the impact of influenza on excess mortality in all ages in Italy during recent seasons (2013/14-2016/17 seasons). Int J Infect Dis. 2019;88:127–34.

    PubMed  Article  Google Scholar 

  29. 29.

    Choi JJ, McCarthy MW. Novel applications for serum procalcitonin testing in clinical practice. Expert Rev Mol Diagn. 2018;18(1):27–34.

    CAS  PubMed  Article  Google Scholar 

  30. 30.

    Lionakis MS, Kontoyiannis DP. Glucocorticoids and invasive fungal infections. Lancet. 2003;362(9398):1828–38.

    CAS  PubMed  Article  Google Scholar 

  31. 31.

    Karthaus M, Buchheidt D. Invasive aspergillosis: new insights into disease, diagnostic and treatment. Curr Pharm Des. 2013;19(20):3569–94.

    CAS  PubMed  Article  Google Scholar 

  32. 32.

    Ni YN, Chen G, Sun J, Liang BM, Liang ZA. The effect of corticosteroids on mortality of patients with influenza pneumonia: a systematic review and meta-analysis. Crit Care. 2019;23(1):99.

    PubMed  PubMed Central  Article  Google Scholar 

  33. 33.

    Li H, Yang SG, Gu L, Zhang Y, Yan XX, Liang ZA, et al. Effect of low-to-moderate-dose corticosteroids on mortality of hospitalised adolescents and adults with influenza A(H1N1)pdm09 viral pneumonia. Influenza Other Respir Viruses. 2017;11(4):345–54.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  34. 34.

    Ghoneim HE, McCullers JA. Adjunctive corticosteroid therapy improves lung immunopathology and survival during severe secondary pneumococcal pneumonia in mice. J Infect Dis. 2014;209(9):1459–68.

    CAS  PubMed  Article  Google Scholar 

  35. 35.

    Osthoff M, Erb S. Neuraminidase inhibitors and influenza infection. JAMA Intern Med. 2016;176(3):415.

    PubMed  Article  Google Scholar 

  36. 36.

    Muthuri SG, Venkatesan S, Myles PR, Leonardi-Bee J, Lim WS, Al Mamun A, et al. Impact of neuraminidase inhibitors on influenza A(H1N1)pdm09-related pneumonia: an individual participant data meta-analysis. Influenza Other Respir Viruses. 2016;10(3):192–204.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  37. 37.

    Zhou W, Li H, Zhang Y, Huang M, He Q, Li P, et al. Diagnostic value of Galactomannan antigen test in serum and Bronchoalveolar lavage fluid samples from patients with Nonneutropenic invasive pulmonary Aspergillosis. J Clin Microbiol. 2017;55(7):2153–61.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  38. 38.

    van de Groep K, Verboom DM, van de Veerdonk FL, Haas P-JA, van der Poll T, Schultz MJ, et al. Detection of invasive Aspergillosis in critically ill patients with influenza: the role of plasma Galactomannan. Am J Respir Crit Care Med. 2019;200(5):636–8.

    PubMed  Article  Google Scholar 

Download references

Acknowledgments

We thank Dr. Bai and Dr. Zhang (Infectious Disease Department, Beijing Jishuitan Hospital) for their valuable comments on the manuscript.

Conflict of interest

None.

Funding

This study is founded by Beijing JST research (ZR-201921).

Author information

Affiliations

Authors

Contributions

Study concept and design: LC, XdH. Acquisition of data: LC, XdH, YlL, CxZ, XqX. Statistical analysis of data: LC. Drafting of the manuscript: LC. Critical revision of the manuscript for important intellectual content: XdH, XqX. All authors agree with the article submission. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Liang Chen.

Ethics declarations

Ethics approval and consent to participate

The study design was approved by the Ethics Committee of Beijing Jishuitan Hospital (No.201911–15). Given the retrospective nature of the study, the Ethics Committee determined that an informed consent was not necessary.

Consent for publication

Not applicable.

Competing interests

The authors declare that they have no competing interests.

Additional information

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplementary information

Additional file 1 : Appendix 1:

Details of Participating centers. Appendix 2 Definition of microbiological criteria of coinfected other pathogens. Appendix 3 Definition of underlying diseases. Appendix 4 Coinfection with other pathogens. Appendix 5 Univariate analysis between the survival group and the deceased group.

Rights and permissions

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Chen, L., Han, X., Li, Y. et al. Invasive pulmonary aspergillosis in immunocompetent patients hospitalised with influenza A-related pneumonia: a multicenter retrospective study. BMC Pulm Med 20, 239 (2020). https://doi.org/10.1186/s12890-020-01257-w

Download citation

Keywords

  • Influenza A
  • Pneumonia
  • Invasive pulmonary aspergillosis
  • Risk factor