Skip to content

Advertisement

BMC Pulmonary Medicine

Open Access
Open Peer Review

This article has Open Peer Review reports available.

How does Open Peer Review work?

Modification of additive effect between vitamins and ETS on childhood asthma risk according to GSTP1 polymorphism : a cross -sectional study

  • So-Yeon Lee1,
  • Bong-Seong Kim2,
  • Sung-Ok Kwon3,
  • Se-Young Oh3,
  • Hye Lim Shin4,
  • Young-Ho Jung5,
  • Eun Lee6,
  • Song-I Yang1,
  • Hyung Young Kim7,
  • Ju-Hee Seo8,
  • Hyo-Bin Kim9,
  • Ji-Won Kwon10,
  • Hae-Ran Lee1Email author and
  • Soo-Jong Hong6Email author
Contributed equally
BMC Pulmonary Medicine201515:125

https://doi.org/10.1186/s12890-015-0093-0

Received: 11 August 2014

Accepted: 4 August 2015

Published: 22 October 2015

Abstract

Background

Asthma is characterized by airway inflammation, and bronchial airways are particularly susceptible to oxidant-induced tissue damage.

Objective

To investigate the effect of dietary antioxidant intake and environmental tobacco smoke (ETS) on the risk of childhood asthma according to genotypes susceptible to airway diseases.

Methods

This cross-sectional study included 1124 elementary school children aged 7–12 years old. Asthma symptoms and smoking history were measured using the International Study of Asthma and Allergies in Childhood (ISAAC) questionnaire. Intake of vitamin A (including retinol and β-carotene), C, and E was measured by a semi-quantitative food frequency questionnaire (FFQ). GSTP1 polymorphisms were genotyped from peripheral blood samples.

Results

ETS was significantly associated with presence of asthma symptoms (adjusted odds ratio [aOR], 2.48; 95 % confidence interval [CI], 1.29–4.76) and diagnosis (aOR, 1.91; 95 % CI, 1.19–3.06). Dietary antioxidant intake was not associated with asthma symptoms, although ETS plus low vitamin A intake showed a significant positive association with asthma diagnosis (aOR, 2.23; 95 % CI, 1.10–4.54). Children with AA at nucleotide 1695 in GSTP1 who had been exposed to ETS and a low vitamin A intake have an increased risk of asthma diagnosis (aOR, 4.44; 95 % CI,1.58–12.52) compared with children who had not been exposed to the two risk factors. However, ETS exposure and low vitamin A intake did not significantly increase odds of asthma diagnosis in children with AG or GG genotypes.

Conclusion

Low vitamin A intake and ETS exposure may increase oxidative stress and thereby risk for childhood asthma. These relationships may be modified by gene susceptibility alleles of GSTP1.

Keywords

AsthmaVitamin AAntioxidantEnvironmental tobacco smokeOxidative stressPolymorphism

Background

Asthma is a chronic inflammatory disease of the respiratory tract, and the bronchial airways are particularly susceptible to oxidation-induced tissue damage [1]. Environmental tobacco smoke (ETS) worsens asthma symptoms and leads to poor asthma control in adults and children [2, 3]. Current guidelines for asthma treatment recommend avoiding exposure to tobacco smoke, including both active smoking and ETS [4]. Children who are exposed to ETS in their homes have lower peak lung function than those who are not exposed [5].

Oxidative stress results in inflammation and tissue damage in the respiratory system and, subsequently, immune system damage. Indeed, individuals with lower cellular reducing capacity have an increased risk of developing asthma [6]. Tobacco smoke is a key source of free radicals related to oxidative stress and therefore increases the risk for asthma [7, 8].

Antioxidants provide protection by preventing oxidative DNA damage [9]. A deficiency in dietary antioxidants is associated with increased asthma risk by increasing susceptibility to oxidative stress [10, 11]. The combination of smoking and low antioxidant levels increase free radicals and therefore lead to antioxidant depletion and oxidative stress [1214]. Smoking is strongly associated with reduced blood concentration of vitamin C, as well as α- and β-carotene [15]. Smoking has also been shown to deplete endogenous antioxidants such as vitamins C and E, β-carotene, ubiquinol, glutathione, and α-lipoic acid [16]. Antioxidant depletion increases individual vulnerability to free radicals and other oxidant species produced by tobacco smoking, and thus increases morbidity, the rate of aging, and risk of death.

Dietary supplementation with antioxidants may reduce the overall oxidative burden that is increased by cigarette smoking [16]. Previous studies indicate that consumption of carotenoids, a group of dietary antioxidants, may reduce the smoking-induced increase in colorectal cancer risk [17]. Another study shows that the intake of nutrients with antioxidant properties may reduce lung function decline in older adults exposed to cigarette smoke [18]. Enhancing antioxidant defenses may reduce the cumulative effects of oxidative damage and perhaps the risk of developing childhood asthma. To the best of our knowledge, no research has evaluated the impact of ETS and dietary antioxidants on the risk of childhood asthma.

Genetic factors, such as gene polymorphisms that alter the antioxidant response, may also contribute to the relationship between asthma and ETS or deficiency of dietary antioxidants. Glutathione S-transferases (GSTs) are a group of enzymes that are reported to neutralize the effects of tobacco smoke and reduce oxidative stress [19]. Most studies investigating genetically associated susceptibility to respiratory abnormalities have focused on GSPT1, the most abundant GST isozyme in the lungs, as well as GSTM1 and GSTT1. The functional sequence variant in GSTP1 at codon 105 (Ile105Val -rs1695) has been associated with asthma in some [20, 21] but not all studies [22]. This variant has been shown to protect against and increase the risk of asthma. Several studies report that GSTP1 genotypes modulate the effect of environment-induced respiratory symptoms and asthma in children [23, 24]. However, the effect of gene polymorphisms on the response to dietary antioxidants and ETS, and subsequently asthma risk, is poorly understood.

We investigated the effect of dietary antioxidant intake and ETS on the presence of asthma symptoms according to GSTP1 polymorphism in children age 7–12 years.

Methods

Study population

All children attending a single elementary school in Seoul, Korea were invited to participate in this study (total 1376 individuals age 7–12 years). Of these, 1356 responded to the questionnaire (response rate 98.6 %). Of the respondents, 1111 children (577 boys and 529 girls) were included in this study. The 245 children who did not answer the food questionnaire were excluded due to insufficient information about their calorie intake. The parents or guardians of all participants signed a written informed consent form. This study was approved by the International Review Board of Asan Medical Center, University of Ulsan.

Questionnaire survey

A Korean version of the International Study of Asthma and Allergies in Childhood (ISAAC) questionnaire and the food frequency questionnaire (FFQ) were completed by each participant’s parents or guardians. The modified Korean version of ISAAC has been validated for assessment of allergy symptoms and diagnosis of allergic diseases in Korean children [25]. Definitions of asthma were based on answers to specific questions, such as “Ever had wheezing?”, “Ever had a doctor’s diagnosis of asthma?”, and “Ever had wheezing during the last 12 months?” [25].

The parents or guardians of each subject were given a questionnaire to complete, which included questions on social background, family history of allergic diseases, and smoking histories. A participant was considered to have exposure to ETS if the answer to the question “Has your child ever been exposed to smoke from tobacco more than once a week?” was “yes.” [26] Frequency of ETS was determined by the answer to the question “If your child was exposed to tobacco smoke more than once a week, how many times was your child exposed to tobacco smoke for a week?”

Dietary intake was assessed by the semi-quantitative FFQ. This questionnaire has been validated previously and includes 113 food items with nine non-overlapping intake frequencies over the preceding year (ranging from “rarely eaten” to “eaten more than 3 times per day”) and three portion sizes (small, average, or large) [27]. Using the Computer Aided Nutritional Analysis Program III (CAN PRO III) developed by the Korean Nutrition Society, the amount of each food item included in the FFQ was converted into grams, from which daily nutrient intake was calculated [28]. Dietary intake of the antioxidant micronutrients vitamins A, C, and E, retinol, and carotene were analyzed.

Serum total IgE levels and pulmonary function test

The concentration of serum total IgE was measured by fluorescent enzyme immunoassay using the AutoCAP System (Phadia AB, Uppsala, Sweden). Data on lung function were collected by trained field technicians who visited the schools. Baseline spirometry was performed for each study participant according to the American Thoracic Society guidelines [29] using a portable microspirometer (Microspiro HI-298; Chest Corporation, Tokyo, Japan). We measured forced vital capacity (FVC), forced expiratory volume in 1 s (FEV1), and the mean forced expiratory flow during the middle half of FVC (FEF25–75%).

Genotyping

Genomic DNA was extracted from peripheral blood of study participants. GSTP1 (rs1695) polymorphisms were genotyped using the TaqMan assay, assay C_3237198_20 (ABI, Foster City, CA, USA) according to the manufacturer’s instructions. End-point readings were obtained using an ABI PRISM 7900 HT Sequence Detection System (ABI).

Statistical analysis

Nutrients were adjusted to total energy intake using the residual method. Adjusted odds ratios (aORs) and 95 % confidence intervals (CIs) were obtained using logistic regression analysis. Statistical analyses were performed using SAS for Windows (version 9.2). Multiple logistic regression analysis was performed by adjusting for key covariates such as age, sex, body mass index (BMI), parental history of allergic diseases, maternal education, log-transformed total energy intake, and monthly household income. A P-value of < 0.05 was considered to indicate statistical significance.

Results

Subject characteristics

Descriptive statistics across the total sample are summarized in Additional file 1: Table S1. Parental history of asthma, maternal education, household income, parental smoking, exposure to ETS, and wheeze in previous 12 months were not significantly different between the included and excluded children. However, the included group of children was younger and had a greater proportion of females, lower BMI, and a greater proportion of children with asthma than the excluded group.

The prevalence of children with an asthma diagnosis was 10.3 %, and 5.8 % of the children had experienced wheezing in the previous 12 months (Table 1). Both wheezing within the previous 12 months and an asthma diagnosis were more prevalent in children exposed to ETS than in those who were not exposed (P = 0.02 and P = 0.04, respectively). Serum IgE level and pulmonary function did not differ between the two groups.
Table 1

Study population characteristics*

Parameter

Total

ETS (+)

ETS (−)

P-value

n

1111

403

708

 

Age (years)

9.48 ± 1.73

9.54 ± 1.68

9.44 ± 1.76

0.384

Sex (male/female)

577/529

366/337

211/192

0.925

BMI

18.46 ± 3.30

18.77 ± 3.48

18.28 ± 3.18

0.024

Parental history of asthma

38/875 (4.3 %)

14/315(4.4 %)

24/560(4.3 %)

0.912

Parental history of AR

347/885 (39.2 %)

109/322(33.9 %)

238/563(42.3 %)

0.014

Parental history of AD

88/896 (9.8 %)

28/323(8.7 %)

60/573(10.5 %)

0.384

Maternal education

Low (≤ high school)

391/1090 (35.9 %)

162/391(41.4 %)

229/699(32.8 %)

0.004

High (> high school)

699/1090 (64.1 %)

229/391(58.6 %)

470/699(67.2 %)

 

Household income (10,000 Korean won)

≤299

312/1055 (29.6 %)

134/386(34.7 %)

178/669(26.6 %)

0.019

 

300–399

292/1055 (27.7 %)

102/386(26.4 %)

190/669(28.4 %)

 
 

≥400

451/1055 (42.8 %)

150/386(38.9 %)

301/669(45.0 %)

 

Paternal smoking

Non-smoker

293/1078 (27.2 %)

34/394(8.6 %)

259/684(37.9 %)

<.0001

Past smoker

263/1078 (24.4 %)

36/394(9.1 %)

227/684(33.2 %)

 

Current smoker

522/1078 (48.4 %)

324/394(82.2 %)

198/684(29.0 %)

 

Maternal smoking

Non-smoker

1075/1091 (98.5 %)

382/391(97.7 %)

693/700(99.0 %)

 

Past smoker

6/1091 (0.6 %)

2/391(0.5 %)

4/700(0.6 %)

0.077

Current smoker

10/1091 (0.9 %)

7/391(1.8 %)

3/700(64.2 %)

 

Frequency of ETS (per week)

≤ twice

 

85/215 (39.5 %)

  
 

2–4 times

 

59/215 (27.4 %)

  
 

≥5 times

 

71/215 (33.0 %)

  

Serum total IgE (kU/L)*

 

58.13 ± 4.01

59.45 ± 3.65

57.38 ± 4.22

0.693

Pulmonary function test*

FVC (%)

89.34 ± 1.13

89.35 ± 1.13

89.33 ± 1.14

0.982

 

FEV1(%)

96.91 ± 1.13

96.86 ± 1.13

96.93 ± 1.13

0.932

 

FEV1/FVC

92.12 ± 1.07

91.99 ± 1.07

92.2 ± 1.06

0.588

 

FEF25–75% (%)

90.63 ± 1.38

91.89 ± 1.31

89.96 ± 1.41

0.554

Wheeze in previous 12 months

 

62/1069 (5.8 %)

31/386 (8.0 %)

31/683 (4.5 %)

0.019

Asthma diagnosis

 

110/1064 (10.3 %)

50/390 (12.8 %)

60/674 (8.9 %)

0.043

AR allergic rhinitis, AD airway disease; BMI body mass index, ETS environmental tobacco smoke, FVC forced vital capacity, FEV1 forced expiratory volume in 1 s, FEF 25–75% forced expiratory flow during the middle half of FVC

*Values are mean ± SD or n (%)

A parental history of asthma was identified in 4.28 % of all subjects. Children who were not exposed to ETS tended to have mothers with higher education levels and come from families with higher household incomes than children exposed to ETS (P = 0.01 for both).

Daily dietary antioxidant intake is described in Table 2. The average intake of total calories and vitamin C were within the dietary reference intake (DRI) range for Koreans. The average vitamin A and E intake was slightly above the DRI for Koreans. Dietary antioxidant intake did not differ between children exposed to ETS and those who were not exposed.
Table 2

Dietary antioxidant intake

 

DRIs for Koreans (children aged 6–14 years)

ETS (+)

ETS (−)

P-value*

Energy (kcal)

1500–1900

1808.31 ± 697.85

1826.91 ± 756.98

0.686

Vitamin A (μg RE)

400–700

712.28 ± 400.25

750.86 ± 479.37

0.151

Retinol (μ)

-

227.88 ± 119.99

221.25 ± 120.26

0.376

Carotene (μg)

-

2719.00 ± 1976.33

2979.41 ± 2487.95

0.055

Vitamin C (mg)

60–100

77.08 ± 62.41

77.05 ± 54.34

0.993

Vitamin E (mg)

7–10

13.40 ± 7.44

13.90 ± 8.46

0.308

DRIs dietary reference intakes, ETS environmental tobacco smoke

Nutrients were adjusted for total energy intake using the residual method

*Nutrient intake in children exposed to ETS exposure versus those not exposed to ETS

Relationship between dietary antioxidant intake and asthma risk

Multivariate analysis showed no association between intake of any of the dietary antioxidants (vitamins A, C, and E, retinol, and carotene) and asthma diagnosis or wheeze within the previous 12 months (Table 3).
Table 3

Associations among antioxidant nutrient intake, environmental tobacco smoke, and prevalence of asthma symptoms

Variable

Wheeze symptom in the previous 12 months

Asthma diagnosis

n (%)

aORf (95 % CI)

n (%)

aORf (95 % CI)

Antioxidant intakeh

Vitamin Aa

Low

46/725 (6.34)

1.00

74/720 (10.28)

1.00

High

17/360 (4.72)

0.53 (0.24, 1.17)

36/362 (9.94)

0.77 (0.44, 1.34)

Retinolb

Low

42/722 (5.82)

1.00

59/714 (8.26)

1.00

High

21/363 (5.79)

0.59 (0.27, 1.28)

51/368 (13.86)

1.51 (0.88, 2.60)

Carotenec

Low

41/725 (5.66)

1.00

71/723 (9.82)

1.00

High

22/360 (6.11)

0.95 (0.46, 2.00)

39/359 (10.86)

0.95 (0.55, 1.62)

Vitamin Cd

Low

38/725 (5.24)

1.00

66/727 (9.08)

1.00

High

25/360 (6.94)

0.97 (0.45, 2.07)

44/355 (12.39)

1.14 (0.66, 1.97)

Vitamin Ee

Low

34/725 (4.69)

1.00

60/726 (8.26)

1.00

High

29/360 (8.06)

1.67 (0.72, 3.87)

50/356 (14.04)

1.57 (0.85, 2.89)

Environmental tobacco smokeg

No

31/683 (4.54)

1.00

60/674 (8.90)

1.00

Yes

31/386 (8.03)

2.48 (1.29, 4.76)*

50/390 (12.82)

1.91 (1.19, 3.06)*

aOR adjusted odds ratio, BMI body mass index

aCut-off point for dietary vitamin A, 803.84 μg/day

bCut-off point for dietary retinol, 253.69 μg/day

cCut-off point for dietary β-carotene, 3081.38 μg/day

dCut-off point for dietary vitamin C, 81.4 mg/day

eCut-off point for dietary vitamin E, 14.68 mg/day

faOR: Adjusted by age, sex, BMI (continuous), parental history of asthma, exposure to environmental tobacco smoke, maternal education, household income, and log-transformed total energy intake

gaOR: Adjusted for the same confounders as above with the exception of environmental tobacco smoke

hNutrients were adjusted for total energy intake

*P-value < 0.01

Relationship between ETS and asthma risk

ETS significantly increased the odds of wheeze in the previous 12 months (aOR, 2.48; 95 % CI, 1.29–4.76) and asthma diagnosis (aOR, 1.91; 95 % CI, 1.19–3.06) (Table 3).

Effects of ETS and dietary antioxidant intake on asthma risk

In a combined analysis of dietary antioxidant intake and ETS, we found an additive effect of a low vitamin A intake and ETS exposure for increasing the risk of asthma diagnosis (Table 4). Among children who were exposed to ETS, those with low dietary intake of vitamin A and retinol had significantly greater odds of reporting wheeze within the previous 12 months than children with high dietary intakes (vitamin A: aOR, 4.43, 95 % CI, 1.51–12.96; retinol: aOR, 5.15, 95 % CI, 1.63–16.25).
Table 4

The combined effects of dietary antioxidant intake and environmental tobacco smoke on the risk for asthma

Variable

Wheezing symptoms in previous 12 months

Asthma diagnosis

N (%)

aORa (95 % CI)

P-value

N (%)

aORa (95 % CI)

P-value

Vitamin A

ETS

          

High

No

6/148(3.90)

1.00

   

19/131(12.67)

1.00

   

Low

No

14/309(4.33)

1.59

(0.54

4.67)

0.40

24/290(7.64)

0.80

(0.39

1.64)

0.55

High

Yes

6/89(6.32)

2.01

(0.61

6.64)

0.25

11/86(11.34)

0.98

(0.44

2.21)

0.96

Low

Yes

17/62(9.50)

4.43

(1.51

12.96)

<0.01

31/152(16.94)

2.23

(1.10

4.54)

0.03

Retinol

ETS

          

High

No

5/149(3.25)

1.00

   

20/134(12.99)

1.00

   

Low

No

15/308(4.64)

2.67

(0.85

8.34)

0.09

23/287(7.42)

0.73

(0.36

1.49)

0.39

High

Yes

8/83(8.79)

4.37

(1.30

14.63)

0.02

19/74(20.43)

2.16

(1.05

4.43)

0.04

Low

Yes

15/168(8.20)

5.15

(1.63

16.25)

0.01

23/164(12.30)

1.29

(0.63

2.66)

0.49

Carotene

ETS

          

High

No

7/144(4.64)

1.00

   

18/127(12.41)

1.00

   

Low

No

13/313(3.99)

0.88

(0.31

2.48)

0.81

25/294(7.84)

0.72

(0.36

1.46)

0.37

High

Yes

8/90(8.16)

2.03

(0.69

6.00)

0.20

13/86(13.13)

1.16

(0.53

2.54)

0.71

Low

Yes

15/161(8.52)

2.45

(0.87

6.85)

0.09

29/152(16.02)

1.84

(0.90

3.73)

0.09

Vitamin C

ETS

          

High

No

7/153(4.38)

1.00

   

21/133(13.64)

1.00

   

Low

No

13/304(4.10)

1.09

(0.38

3.15)

0.87

22/288(7.10)

0.58

(0.28

1.18)

0.13

High

Yes

9/90(9.09)

2.65

(0.92

7.64)

0.07

14/86(14.00)

1.13

(0.53

2.40)

0.75

Low

Yes

14/161(8.00)

2.61

(0.90

7.59)

0.08

28/152(15.56)

1.55

(0.76

3.13)

0.23

Vitamin E

ETS

          

High

No

9/166(5.14)

1.00

   

23/145(13.69)

1.00

   

Low

No

11/291(3.64)

0.78

(0.26

2.31)

0.65

20/276(6.76)

0.56

(0.26

1.21)

0.14

High

Yes

11/84(11.58)

3.32

(1.25

8.80)

0.02

18/81(18.18)

1.67

(0.83

3.36)

0.15

Low

Yes

12/167(6.70)

1.58

(0.54

4.64)

0.41

24/157(13.26)

1.22

(0.57

2.58)

0.61

ETS environmental tobacco smoke

aaOR: Adjusted for age, sex, BMI (continuous), parental history of asthma, maternal education, household income, and log-transformed total energy intake

Effect of ETS and dietary antioxidant intake on asthma risk according to GSTP1 polymorphism

The distribution of GSTP1 polymorphism was in Hardy–Weinberg equilibrium. The number and proportion of subjects with the AA, AG, and GG GSTP1 polymorphisms were 597 (62.6 %), 319 (33.4 %), and 38 (4.0 %), respectively. The Hardy-Weinberg equilibrium P-value for this polymorphism was 0.569. The relationship between dietary antioxidant intake and ETS was more apparent in children with AA at nucleotide 1695 of the GSTP1. Children with the AA genotype who had been exposed to ETS and had low intakes of vitamin A or carotene were more likely to have an asthma diagnosis than children with no ETS exposure and high intakes of vitamin A or carotene (vitamin A: aOR, 4.44, 95 % CI, 1.58–12.52; carotene: aOR, 3.15, 95 % CI, 1.15–8.63; Additional file 2: Table S2, Figs. 1 and 2). However, low vitamin intake and ETS exposure did not significantly increase the odds of asthma diagnosis in children with AG or GG genotypes.
Fig. 1

Combined effects of vitamin A and environmental tobacco smoke (ETS) on the risk of asthma diagnosis regarding glutathione S-transferase P1 (GSTP1) polymorphism

Fig. 2

Combined effects of carotene and environmental tobacco smoke (ETS) on the risk of asthma diagnosis regarding glutathione S-transferase P1 (GSTP1) polymorphism

In children with an AA genotype, children with ETS and a high retinol intake were more likely to have an asthma diagnosis (aOR, 4.18, 95 % CI, 1.51–11.57; Additional file 1: Table S1), although this association was not observed in children with the AG or GG genotypes.

Discussion

This study showed an additive effect of low dietary intake of vitamin A and ETS exposure for increasing risk for asthma symptoms. Additionally, the AA GSTP1 polymorphism was associated with an increased risk for asthma in children who were exposed to ETS and had a low dietary intake of vitamin A and carotene. Children who were exposed to ETS were significantly more likely to report wheeze within the previous 12 months and have an asthma diagnosis. Although overall antioxidant intake was not associated with presence of asthma symptoms, children who were exposed to ETS and had a low vitamin A intake were more likely to report asthma symptoms. This trend was particularly notable in children carrying the GSTP1 genotype AA, which has been associated with an increased risk for asthma. Our study suggested that low vitamin A intake increased susceptibility to development of ETS-associated childhood asthma by decreasing antioxidant capacity, and the oxidative stress-related GSTP1 gene further modified this association.

Oxidative stress occurs when the generation of oxidant molecules (i.e., free radicals) exceeds the available antioxidant defenses [30]. Inflammatory disorders such as asthma and allergic rhinitis may be mediated by oxidative stress [25], which occurs as a result of endogenous inflammation and following environmental exposure to toxic substances such as cigarette smoke and air pollutants in allergic airway diseases [6]. Exposure to ETS is a major environmental factor that influences the development and aggravation of asthma and impaired lung function in childhood [7].

Cigarette smoke inhalation increases exposure to reactive oxygen species [31] to a level that may overwhelm endogenous antioxidant defenses in asthmatic patients who already have exacerbated levels of oxidative stress [6]. A controlled human exposure model has shown that glutathione levels are reduced in the bronchial and nasal airways following exposure to air pollutants [32]. The body produces numerous antioxidants endogenously, but the quantity is often insufficient to prevent oxidative stress. Exogenous antioxidants, such as dietary nutrients, can supplement the endogenous system to help defend against free radicals. Smoking is associated with reduced circulating concentration of antioxidants in the blood [33]. This may be because dietary and supplemental antioxidant intake tends to be lower in smokers than in non-smokers and because smoke-induced oxidative stress increases the degradation or transformation of circulating antioxidant micronutrients into biologically inactive components [34] or even into pro-oxidants [35]. Therefore, dietary antioxidant intake may influence the relationship between exposure to ETS and the risk of asthma symptoms.

Several studies suggest that low serum levels or dietary intake of antioxidants may be risk factors for asthma [9, 10]. Dietary vitamin A intake and serum vitamin A concentrations are significantly lower in patients with asthma than in healthy control subjects and are lower in patients with severe asthma than in those with mild asthma [36, 37]. One study shows that low vitamin A status increases susceptibility to cigarette smoke-induced lung emphysema in a mouse model [38]. However, other studies suggest that dietary supplementation with vitamins, such as vitamin A and ascorbate, does not improve lung function or asthma symptoms [3941]. We found no relationship between overall dietary antioxidant intake and asthma diagnosis or wheeze in the previous 12 months, although children who were exposed to ETS and had a low dietary vitamin A intake were more likely to report symptoms of asthma. Dietary antioxidants may ameliorate the effects of smoking on asthma symptoms, although future human studies that assess the benefits of antioxidant intake should focus on selecting an appropriate exposure to oxidative stress.

Recent studies suggest that genetic factors may also contribute to an individual’s susceptibility to respiratory disorders induced by ETS exposure [7]. GSTP1 encodes for an enzyme that belongs to a large family of GST enzymes, which are important for detoxification of potentially harmful compounds from tobacco smoke, such as the polyaromatic hydrocarbon molecules benzopyrene and chrysene [42, 43]. GSTP1 is widely expressed in human airways, predominantly in alveolar macrophages and epithelial cells [44]. Polymorphisms in the GST genes, such as GSPT1 (rs1695), affect the ability to respond to excessive oxidative stress by altering activity of the GST enzymes [45]. Based on the hypothesis that dietary intake of antioxidants and endogenous antioxidant capacity contribute to the susceptibility to oxidative stress in asthmatic children, researchers investigated the effects of antioxidant supplementation on ozone-related decreases in lung function according to GSTM1 genotype [46, 47]. Children in the placebo group that lacked the GSTM1 gene had a significant reduction in FEF25–75% after ozone exposure, whereas the GSTM1-positive children in the placebo group did not. Therefore, asthmatic children with compromised antioxidant defense systems caused by genetic susceptibility and deficiencies in antioxidant intake may be at increased risk for oxidative stress induced by ozone or ETS. However, a recent meta-analysis indicates that the GSTP1 single nucleotide polymorphism rs1695 did not affect the prevalence of asthma, suggesting that presence of GST variants contribute to airway diseases through interactions with the environment [48].

Active GSTP1 variant proteins produced by the GSTP1 gene play a role in xenobiotic metabolism and influence susceptibility to asthma and other diseases [49]. Some studies show that GSTP1 encodes for an important enzyme in the anti-oxidative pathway that buffers the harmful effects of air pollution [21, 50]. The interaction between GSTP1 and different types of air pollutants has a higher information gain than other gene-air pollutant combinations [21]. Therefore, we investigated the influence of interactions between ETS, dietary antioxidant intake, and the GSTP1 gene on risk for childhood asthma. We also investigated the relationship between MTHFR (rs1801133) and NQO1 (rs1800566) genes and asthma symptoms, but we did not present these data because we did not find an association.

To the best of our knowledge, few publications have investigated the overall effects of GST variants and ETS exposure on asthma symptoms [5155], and whether such effects could be modulated by dietary antioxidant intake has not yet been explored. This was the first study to assess how ETS, low dietary vitamin A intake, and GSTP1 genotype affect asthma symptoms in children. However, our study had some limitations. First, this was a cross-sectional study, and therefore we could not determine causal relationships among the factors studied. Second, we focused on only one well-known candidate gene involved in oxidative stress, and other genes likely also regulate the influence of ETS and dietary antioxidants. Third, our study may also have recall bias because our dietary data were based on the semi-quantitative FFQ completed by parents or guardians, who may have underreported unhealthy foods and overreported healthy foods. Fourth, the number of children in the asthma group was smaller than the number in the control group, a discrepancy that is common in community-based studies. In addition, we did not record the use of other supplements, such as multivitamins, and we could not confirm the association between dietary intake and serum levels of antioxidants because we did not measure serum levels. Nonetheless, the clinical implications of these findings are important because exposure to ETS is common in children. Further prospective, long-term follow-up studies are needed to confirm and extend these findings.

Conclusion

Our data showed that low dietary intake of vitamin A and exposure to ETS may increase oxidative stress, which may increase the risk of asthma in children. These relationships may be shaped further by genetic susceptibility alleles of GSTP1.

Notes

Declarations

Acknowledgment

This study was supported by a grand from the Korea Healthcare technology R&D Project, Ministry for Health, Welfare, Republic of Korea (A092076).

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

Authors’ Affiliations

(1)
Department of Pediatrics, Hallym Sacred Heart Hospital, Hallym University College of Medicine, Dongan-gu, Anyang, South Korea
(2)
Department of Pediatrics, Gangneung Asan Hospital, University of Ulsan College of Medicine, Gangneung, South Korea
(3)
Department of Food and Nutrition, College of Human Ecology, Kyung Hee University, Seoul, South Korea
(4)
Research Center for Standardization of Allergic Diseases, Asan Institute for Life Sciences, University of Ulsan College of Medicine, Seoul, South Korea
(5)
Department of Pediatrics, CHA Bundang Medical Center, CHA University School of Medicine, Seongnam, South Korea
(6)
Department of Pediatrics, Childhood Asthma Atopy Center, Asan Medical Center, University of Ulsan College of Medicine, Songpa-gu, South Korea
(7)
Department of Pediatrics, Kosin University Gospel Hospital, Busan, South Korea
(8)
Department of Pediatrics, Korea Cancer Center Hospital, Seoul, South Korea
(9)
Department of Pediatrics, Sanggye Paik Hospital, Inje University College of Medicine, Seoul, South Korea
(10)
Department of Pediatrics, Seoul National University Bundang Hospital, Seongnam, South Korea

References

  1. Dworski R. Oxidant stress in asthma. Thorax. 2000;55 Suppl 2:51–3.View ArticleGoogle Scholar
  2. Pietinalho A, Pelkonen A, Rytila P. Linkage between smoking and asthma. Allergy. 2009;64:1722–7.View ArticlePubMedGoogle Scholar
  3. Lazarus SC, Chinchilli VM, Rollings NJ, Boushey HA, Cherniack R, Craig TJ, et al. Smoking affects response to inhaled corticosteroids or leukotriene receptor antagonists in asthma. Am J Respir Crit Care Med. 2007;175:783–90.View ArticlePubMedPubMed CentralGoogle Scholar
  4. National Asthma Education and Prevention Program, Third Expert Panel on the Management of Asthma. Guidelines for the Diagnosis and Management of Asthma: Full Report 2007. Bethesda, MD: US Department of Health and Human Services, National Institutes of Health, National Heart, Lung, and Blood Institute; 2007.Google Scholar
  5. He QQ, Wong TW, Du L, Jiang ZQ, Yu TS, Qiu H, et al. Environmental tobacco smoke exposure and Chinese schoolchildren's respiratory health: a prospective cohort study. Am J Prev Med. 2011;41:487–93.View ArticlePubMedGoogle Scholar
  6. Bowler RP, Crapo JD. Oxidative stress in allergic respiratory diseases. J Allergy Clin Immunol. 2002;110:349–56.View ArticlePubMedGoogle Scholar
  7. Munoz B, Magana JJ, Romero-Toledo I, Juárez-Pérez E, López-Moya A, Lewa-Garcia N, et al. The relationship among IL-13, GSTP1, and CYP1A1 polymorphisms and environmental tobacco smoke in a population of children with asthma in Northern Mexico. Environ Toxicol Pharmacol. 2012;33:226–32.View ArticlePubMedGoogle Scholar
  8. Dozor AJ. The role of oxidative stress in the pathogenesis and treatment of asthma. Ann N Y Acad Sci. 2010;1203:133–7.View ArticlePubMedGoogle Scholar
  9. Marmsjö K, Rosenlund H, Kull I, Hakansson N, Wickman M, Pershagen G, et al. Use of multivitamin supplements in relation to allergic disease in 8-y-old children. Am J Clin Nutr. 2009;90:1693–8.View ArticlePubMedGoogle Scholar
  10. Powell CV, Nash AA, Powers HJ, Primhak RA. Antioxidant status in asthma. Pediatr Pulmonol. 1994;18:34–8.View ArticlePubMedGoogle Scholar
  11. Vural H, Uzun K. Serum and red blood cell antioxidant status in patients with bronchial asthma. Can Respir J. 2000;7:476–80.View ArticlePubMedGoogle Scholar
  12. Chelchowska M, Ambroszkiewicz J, Gajewska J, Laskowska-Klita T, Leibschang J. The effect of tobacco smoking during pregnancy on plasma oxidant and antioxidant status in mother and newborn. Eur J Obstet Gynecol Reprod Biol. 2011;155:132–6.View ArticlePubMedGoogle Scholar
  13. Aycicek A, Erel O, Kocyigit A. Increased oxidative stress in infants exposed to passive smoking. Eur J Pediatr. 2005;164:775–8.View ArticlePubMedGoogle Scholar
  14. Orhon FS, Ulukol B, Kahya D, Cengiz B, Baskan S, Tezcan S. The influence of maternal smoking on maternal and newborn oxidant and antioxidant status. Eur J Pediatr. 2009;168:975–81.View ArticlePubMedGoogle Scholar
  15. Wei W, Kim Y, Boudreau N. Association of smoking with serum and dietary levels of antioxidants in adults: NHANES III, 1988–1994. Am J Public Health. 2001;91:258–64.View ArticlePubMedPubMed CentralGoogle Scholar
  16. Elsayed NM, Bendich A. Dietary antioxidants: potential effects on oxidative products in cigarette smoke. Nutrition Research. 2001;21:551–67.View ArticleGoogle Scholar
  17. Chaiter Y, Gruber SB, Ben-Amotz A, Ben-Amotz A, Almog R, Rennert HS, et al. Smoking attenuates the negative association between carotenoids consumption and colorectal cancer risk. Cancer Causes Control. 2009;20:1327–38.View ArticlePubMedGoogle Scholar
  18. Bentley AR, Kritchevsky SB, Harris TB, Holvoet P, Jensen RL, Newman AB, et al. Dietary antioxidants and FEV1 decline: the health, aging and body composition study. Eur Respir J. 2012;39:979–84.View ArticlePubMedGoogle Scholar
  19. Spiteri MA, Bianco A, Strange RC, Fryer AA. Polymorphisms at the glutathione S-transferase, GSTP1 locus: a novel mechanism for susceptibility and development of atopic airway inflammation. Allergy. 2000;55:15–20.View ArticlePubMedGoogle Scholar
  20. Fryer AA, Bianco A, Hepple M, Jones PW, Strange RC, Spiteri MA. Polymorphism at the glutathione S-transferase GSTP1 locus: a new marker for bronchial hyperresponsiveness and asthma. Am J Respir Crit Care Med. 2000;161:1437–42.View ArticlePubMedGoogle Scholar
  21. Su MW, Tsai CH, Tung KY, Hwang BF, Liang PH, Chiang BL, et al. GSTP1 is a hub gene for gene-air pollution interactions on childhood asthma. Allergy. 2013;68:1614–7.View ArticlePubMedGoogle Scholar
  22. Mak JC, Ho SP, Leung HC, Cheung AH, Law BK, So LK, et al. Relationship between glutathione S-transferase gene polymorphisms and enzyme activity in Hong Kong Chinese asthmatics. Clin Exp Allergy. 2007;37:1150–7.View ArticlePubMedGoogle Scholar
  23. Schultz EN, Devadason SG, Khoo SK, Zhang G, Bizzintino JA, Martin AC, et al. The role of GSTP1 polymorphisms and tobacco smoke exposure in children with acute asthma. J Asthma. 2010;47:1049–56.View ArticlePubMedGoogle Scholar
  24. Islam T, Berhane K, McConnell R, Gauderman WJ, Avol E, Peters JM, et al. Glutathione-S-transferase (GST) P1, GSTM1, exercise, ozone and asthma incidence in school children. Thorax. 2009;64:197–202.View ArticlePubMedGoogle Scholar
  25. Hong SJ, Lee MS, Sohn MH, Shim JY, Han YS, Park KS, et al. Self-reported prevalence and risk factors of asthma among Korean adolescents: 5-year follow-up study, 1995–2000. Clin Exp Allergy. 2004;34:1556–62.View ArticlePubMedGoogle Scholar
  26. Yoo S, Kim HB, Lee SY, Kim BS, Kim JH, Yu J, et al. Effect of active smoking on asthma symptoms, pulmonary function, and BHR in adolescents. Pediatr Pulmonol. 2009;44:954–61.View ArticlePubMedGoogle Scholar
  27. Oh SY, Kim EM, Shin MHL, Lee SH, Kim JE, Lee HS. Development and validation of food frequency questionnaire for adults. Seoul, Korea: The Korean Society of Health Promotion Annual Spring Conference; 2007. p. 67–72.Google Scholar
  28. Seo JH, Kwon SO, Lee SY, Kim HY, Kwon JW, Kim BJ, et al. Association of antioxidants with allergic rhinitis in children from Seoul. Allergy Asthma Immunol Res. 2013;5:81–7.View ArticlePubMedGoogle Scholar
  29. American Thoracic Society. Standardization of spirometry: 1994 update. Am J Respir Crit Care Med. 1995;152:1107–36.View ArticleGoogle Scholar
  30. Canova C, Dunster C, Kelly FJ, Minelli C, Shah PL, Caneja C, et al. PM10-induced hospital admissions for asthma and chronic obstructive pulmonary disease: the modifying effect of individual characteristics. Epidemiology. 2012;23:607–15.View ArticlePubMedGoogle Scholar
  31. Winston GW, Church DF, Cueto R, Pryor WA. Oxygen consumption and oxyradical production from microsomal reduction of aqueous extracts of cigarette tar. Arch Biochem Biophys. 1993;304:371–8.View ArticlePubMedGoogle Scholar
  32. Mudway IS, Stenfors N, Duggan ST, Roxborough H, Zielinski H, Marklund SL, et al. An in vitro and in vivo investiation of the effects of diesel exhaust on human airway lining fluid antioxidants. Arch Biochem Biophys. 2004;423:200–12.View ArticlePubMedGoogle Scholar
  33. Aoki K, Ito Y, Sasaki R, Ohtani M, Hamajima N, Asano A. Smoking, alcohol drinking and serum carotenoids levels. Jpn J Cancer Res. 1987;78:1049–56.PubMedGoogle Scholar
  34. van den Berg H, van der Gaag M, Hendriks H. Influence of lifestyle on vitamin bioavailability. Int J Vitam Nutr Res. 2002;72:53–9.View ArticlePubMedGoogle Scholar
  35. Palozza P, Serini S, Di Nicuolo F, Boninsegna A, Torsello A, Maggiano N, et al. ß-carotene exacerbates DNA oxidative damage and modifies p53-related pathways of cell proliferation and apoptosis in cultured cells exposed to tobacco smoke condensate. Carcinogenesis. 2004;25:1315–25.View ArticlePubMedGoogle Scholar
  36. Allen S, Britton JR, Leonardi-Bee JA. Association between antioxidant vitamins and asthma outcome measures: systematic review and meta-analysis. Thorax. 2009;64:610–9.View ArticlePubMedGoogle Scholar
  37. Al Senaidy AM. Serum vitamin A and beta-carotene levels in children with asthma. J Asthma. 2009;46:699–702.View ArticlePubMedGoogle Scholar
  38. van Eijl S, Mortaz E, Versluis C, Nijkamp FP, Folkerts G, Bloksma N. A low vitamin A status increases the susceptibility to cigarette smoke-induced lung emphysema in C57BL/6 J mice. J Physio Pharmacol. 2011;62:175–82.Google Scholar
  39. Ting S, Mansfield LE, Yarbrough J. Effects of ascorbic acid on pulmonary functions in mild asthma. J Asthma. 1983;20:39–42.View ArticlePubMedGoogle Scholar
  40. Kaur B, Rowe BH, Ram FS. Vitamin C supplementation for asthma. Cochrane Database Syst Rev. 2001;CD000993.Google Scholar
  41. Grievink L, Smit HA, Ocké MC, van't Veer P, Kromhout D. Dietary intake of antioxidant (pro)-vitamins, respiratory symptoms and pulmonary function: the MORGEN Study. Thorax. 1998;53:166–71.View ArticlePubMedPubMed CentralGoogle Scholar
  42. Hu X, Xia H, Srivastava SK, Herzog C, Awasthi YC, Ji X, et al. Activity of four allelic forms of glutathione S-transferase hGSTP1-1 for diol epoxides of polycyclic aromatic hydrocarbons. Biochem Biophys Res Commun. 1997;238:397–402.View ArticlePubMedGoogle Scholar
  43. Hoffmann D, Hoffmann I, El-Bayoumy K. The less harmful cigarette: a controversial issue. a tribute to Ernst L. Wynder. Chem Res Toxicol. 2001;14:767–90.View ArticlePubMedGoogle Scholar
  44. Hoskins A, Wu P, Reiss S, Dworski R. Glutathione S-transferase P1 Ile105Val polymorphism modulates allergen-induced airway inflammation in human atopic asthmatics in vivo. Clin Exp Allergy. 2013;43:527–34.View ArticlePubMedPubMed CentralGoogle Scholar
  45. Hayes JD, Strange RC. Glutathione S-transferase polymorphisms and their biological consequences. Pharmacology. 2000;61:154–66.View ArticlePubMedGoogle Scholar
  46. Romieu I, Sienra-Monge JJ, Ramírez-Aguilar M, Moreno-Macias H, Reyes-Ruiz NI, Estela del Rio-Navaro B, et al. Genetic polymorphism of GSTM1 and antioxidant supplementation influence lung function in relation to ozone exposure in asthmatic children Mexico City. Thorax. 2004;59:8–10.PubMedPubMed CentralGoogle Scholar
  47. Moreno-Macías H, Dockery DW, Schwartz J, Gold DR, Laird NM, Sienra-Monge JJ, et al. Ozone exposure, vitamin C intake, and genetic susceptibility of asthmatic children in Mexico City: a cohort study. Respir Res. 2013;14:14–24.View ArticlePubMedPubMed CentralGoogle Scholar
  48. Minelli C, Granell R, Newson R, Rose-Zerilli MJ, Torrent M, Ring SM, et al. Shaheen So, Henderson JA: Glutathione-S-transferase genes and asthma phenotypes: a Human Genome Epidemiology (HuGE) systematic review and meta-analysis including unpublished data. Int J Epidemiol. 2010;39:539–62.View ArticlePubMedGoogle Scholar
  49. Mapp CE, Fryer AA, De Marzo N, Pozzato V, Padoan M, Boschetto P, et al. Glutathione S-transferase GSTP1 is a susceptibility gene for occupational asthma induced by isocyanates. J Allergy Clin Immunol. 2002;109:867–72.View ArticlePubMedGoogle Scholar
  50. Melén E, Nyberg F, Lindgren CM, Berglind N, Zucchelli M, Nording E, et al. Interactions between glutathione S-transferase P1, tumor necrosis factor, and traffic-related air pollution for development of childhood allergic disease. Environ Health Perspect. 2008;116:1077–84.View ArticlePubMedPubMed CentralGoogle Scholar
  51. Gilliland FD, Li YF, Dubeau L, Berhane K, Avol E, McConnell R, et al. Effects of glutathione S-transferase M1, maternal smoking during pregnancy, and environmental tobacco smoke on asthma and wheezing in children. Am J Respir Crit Care Med. 2002;166:457–63.View ArticlePubMedGoogle Scholar
  52. Kabesch M, Hoefler C, Carr D, Leupold W, Weiland SK, von Mutius E. Glutathione S transferase deficiency and passive smoking increase childhood asthma. Thorax. 2004;59:569–73.View ArticlePubMedPubMed CentralGoogle Scholar
  53. Lee YL, Lee YC, Guo YL. Associations of glutathione S-transferase P1, M1, and environmental tobacco smoke with wheezing illness in school children. Allergy. 2007;62:641–7.View ArticlePubMedGoogle Scholar
  54. Schroer KT, Biagini Myers JM, Ryan PH, LeMasters GK, Bernstein DI, Villareal M, et al. Associations between multiple environmental exposures and Glutathione S-Transferase P1 on persistent wheezing in a birth cohort. J Pediatr. 2009;154:401–8.View ArticlePubMedGoogle Scholar
  55. Wu J, Hankinson J, Kopec-Harding K, Custovic A, Simpson A. Interaction between glutathione S-transferase variants, maternal smoking and childhood wheezing changes with age. Pediatr Allergy Immunol. 2013;24:501–8.View ArticlePubMedGoogle Scholar

Copyright

© Lee et al. 2015

Advertisement